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Plasmid#112267PurposeITRACK Acceptor ConstructDepositorInsertmCherry with KRas membrane targeting domain
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
UseTags6xHis and CAAXExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-GR-AKAR3
Plasmid#173016PurposePlasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.DepositorInsertLyn-GR-AKAR3
UseTagsEGFP, N-terminal targeting sequence from Lyn kina…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1-Lck-GCaMP2
Plasmid#24794DepositorInsertLck-GCaMP2
UseTagsHis and XpressExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
Myc-CD4-mCherry-HA-FRB
Plasmid#100740PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD4
UseTagsFRB, HA, Myc, and mCherryExpressionMammalianMutationEngineered to remove large part of extracellular …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
PM-APEX2
Plasmid#215557PurposeStable, inducible expression in plasma membraneDepositorInsertLyn11-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-ExRai-AMPKAR
Plasmid#192449PurposeExRai-AMPKAR targeted to the lysosomal membrane.DepositorInsertLyso-ExRai-AMPKAR
UseTagsFull-length LAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-ExRai-AktAR2
Plasmid#184050PurposePlasma membrane-targeted ExRai-AktAR2DepositorInsertPM-ExRai-AktAR2
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-OMP25
Plasmid#141149PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorInsertOMP25 (Synj2bp Budding Yeast, Rat)
UseTagsSNAP tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Sec61B
Plasmid#141152PurposeFluorescent labeling of ER membrane in mammalian cellsDepositorInsertSec61 beta (SEC61B Human)
UseTagsSNAP tagExpressionMammalianMutationL79M on Sec61BPromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only