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pEPOR1CB0014

Plasmid

#117550

Purpose

Level 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0019

Plasmid

#117529

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.1 (no stop codon)

Use

CRISPR

Mutation

K848A, K1003A, R1060A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0017

Plasmid

#117527

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.0 (no stop codon)

Use

CRISPR

Mutation

K810A, K1003A, R1060A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0050

Plasmid

#117617

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0051

Plasmid

#117618

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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GST-GNU-N

Plasmid

#112972

Purpose

Expresses fusion of GST and N terminal amino acids of GNU

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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gnu cDNA LD12084

Plasmid

#112973

Purpose

Cloning vector carrying the gnu cDNA

Depositor

Terry Orr-Weaver

Article

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCK26

Plasmid

#105429

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Bs3

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM16841

Plasmid

#105416

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

STOP codon and nos terminator

Use

Synthetic Biology

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG267

Plasmid

#105339

Purpose

Synthetic biology; gateway destination vector assembly

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-MTS2 (12)

Plasmid

#101698

Purpose

MTS2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-TDH2t (17-21)

Plasmid

#101702

Purpose

TDH2t in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YJR009C (136 bp)]-CGCT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-HygroBR (01-21)

Plasmid

#101704

Purpose

HygroBR in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG [HygroBR (loxP-PAgTEF-hph-TAgTEF-loxP)] CGCT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pGAL1 (01-11)

Plasmid

#101682

Purpose

pGAL1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YBR020W (455bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pTDH3 (01-11)

Plasmid

#101683

Purpose

pTDH3 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YGR192C (675bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pTDH2 (01-11)

Plasmid

#101684

Purpose

pTDH2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YJR009C (699bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pHXT7 (01-11)

Plasmid

#101686

Purpose

pHXT7 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YDR342C (587bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pTPI1 (01-11)

Plasmid

#101687

Purpose

pTPI1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YDR050C (918bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pPYK1 (01-11)

Plasmid

#101688

Purpose

pPYK1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YAL038W (727bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-PGI1 (01-11)

Plasmid

#101690

Purpose

pPGI1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YBR196C (402bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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