We narrowed to 2,973 results for: GAI

1. pENTR U6-hTERTsh

   Plasmid

   #111385

   Purpose

   Knock down of hTERT transcript

   Depositor

   Klaus Holzmann

   Article

   Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

   Insert

   shRNA C3 against hTERT
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB700-mouse-ACTC1-Puro

   Plasmid

   #128326

   Purpose

   SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)

   Depositor

   Alejandro Chavez

   Insert

   gRNA against mouse ACTC1 for activation (Actc1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

   Plasmid

   #118158

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mSprouty1/3B14

   Plasmid

   #22091

   Depositor

   Gail Martin

   Article

   Minowada et al Development. 1999 Oct . 126(20):4465-75.

   Insert

   Sprouty 1 (Spry1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

5. 3ME6/mSprouty2

   Plasmid

   #22097

   Depositor

   Gail Martin

   Article

   Minowada et al Development. 1999 Oct . 126(20):4465-75.

   Insert

   Sprouty 2 (Spry2 Mouse)
   

   Use

   Bacterial

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pTALYM4SpMi-01

   Plasmid

   #47879

   Purpose

   TALE-mRuby2 against Mus spretus minor satellite sequence containing two SNPs

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

   Insert

   TALE-mRuby2
   

   Use

   TALEN

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. cFGF8

   Plasmid

   #22089

   Depositor

   Gail Martin

   Article

   Crossley et al Cell. 1996 Jan 12. 84(1):127-36.

   Insert

   FGF8 (FGF8 Chicken)
   

   Use

   Bacterial

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

8. PCTK1

   Plasmid

   #39053

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PCTK1 (CDK16 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Activation loop mutant S319D

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGKO1

   Plasmid

   #63611

   Purpose

   a binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.

   Depositor

   Seogchan Kang

   Article

   Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

   Type

   Empty backbone

   Use

   Binary fungal vector

   Tags

   Expression

   Mutation

   Promoter

   Lac Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLfa PUb TAL-KMO-R

    Plasmid

    #52888

    Purpose

    Right hand TALEN against Aedes aegypti KMO gene

    Depositor

    Zach Adelman

    Article

    Aryan et al PLoS One. 2013;8(3):e60082. doi: 10.1371

    Insert

    TALEN-KMO
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ae aegypti polyubiquitin

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124288

    Purpose

    sgRNA against human NFATc3

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    NFATc3 nuclear factor of activated T cells (NFATC3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

12. pKG136

    Plasmid

    #63075

    Purpose

    Retroviral expression of EGFP-TEV-S-tag CENP-W for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-W (CENPW Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    E. coli codon optimized, hardened against Dharmac…

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3CB1/cSprouty2

    Plasmid

    #22096

    Depositor

    Gail Martin

    Article

    Minowada et al Development. 1999 Oct . 126(20):4465-75.

    Insert

    Sprouty 2 (SPRY2 Chicken)
    

    Use

    Bacterial

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pSuper 44-2

    Plasmid

    #51598

    Purpose

    shRNA 2 against rat Sema4D

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    shRNA2
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid

    #214688

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Puromycin resistance cassette has silent mutation…

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDual_dsCas9_Puro_hs_TP73

    Plasmid

    #214689

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Puromycin resistance cassette has silent mutation…

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid

    #118159

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid

    #118160

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSuper-PlexinB2

    Plasmid

    #115175

    Purpose

    shRNA against rodent PlexinB2

    Depositor

    Suzanne Paradis

    Article

    McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

    Insert

    shRNA targeting Plxnb2
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN10-gDM1d

    Plasmid

    #114387

    Purpose

    pPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGG

    Depositor

    Vincent Dion

    Article

    Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

    Insert

    gDM1d
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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