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Showing: 2281 - 2300 of 3333 results

  1. pJYDN2p

    Plasmid
    #162455
    Purpose
    Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-DnbALFA (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA nanobody and HA and myc
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162455-plasmid-map-sequence-id-321462

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  6. pBUN276

    Plasmid
    #60673
    Purpose
    overexpression of M. tuberculosis ddl gene in Mycobacterium
    Depositor
    Raul Barletta
    Article
    Feng et al Antimicrob Agents Chemother. 2003 Jan;47
    Insert
    ddl
    Use
    E.coli-mycobacterium shuttle vector
    Tags
    6 codons of M. bovis BCG hsp60
    Expression
    Mutation
    Promoter
    BCG Hsp60
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site

    Plasmid
    #228633
    Purpose
    Bacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)
    Depositor
    Frank von Delft, ASAP Discovery
    Insert
    Enterovirus Coxsackievirus A71 2A protease
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    C110A
    Promoter
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228633-plasmid-map-sequence-id-464103

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  8. pJB72

    Plasmid
    #226312
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with Y to A CsoS2 MR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y300A
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226312-plasmid-map-sequence-id-449924

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  9. pJB44

    Plasmid
    #226303
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with C to S MR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C292S, C310S
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226303-plasmid-map-sequence-id-449915

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  10. pJB43

    Plasmid
    #226302
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    WT CsoS2, frameshift site intact
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226302-plasmid-map-sequence-id-449914

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  13. pYPQ301

    Plasmid
    #205415
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoter
    Depositor
    Yiping Qi
    Article
    Li et al Front Genome Ed. 2023 Oct 12;5:1251903.
    Insert
    Hs1Cas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    205415-plasmid-map-sequence-id-414100

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  14. pSM-dCas9::Ap

    Plasmid
    #154307
    Purpose
    Shuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    I-Son Ng
    Article
    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154307-plasmid-map-sequence-id-306378

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  18. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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Showing: 2281 - 2300 of 3333 results