We narrowed to 8,358 results for: BLI;

1. hsKIF5B(1-560)-HaloTag-FRB

   Plasmid

   #213899

   Purpose

   Imaging of KIF5B fused to HaloTag

   Depositor

   Lukas Kapitein

   Insert

   hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pD454-SR mouse Alpha-synuclein

   Plasmid

   #89075

   Purpose

   bacterial expression of mouse Alpha-synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Alpha-synuclein (Snca Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 17, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pET-24a-ELP[V-30]

   Plasmid

   #67014

   Purpose

   Expresses ELP[V-30] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   ELP[V-30]
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. hGli2 flag3x

   Plasmid

   #84920

   Purpose

   mammalian expression of Gli2

   Depositor

   Martin Fernandez-Zapico

   Insert

   Gli2 (GLI2 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. human EB1 fused to monomeric RFP

   Plasmid

   #39323

   Depositor

   Jennifer Tirnauer, Tim Mitchison

   Inserts

   EB1 (MAPRE1 Human)
   mRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLKO-Tet-ON-shCtrl (neo)

   Plasmid

   #192076

   Purpose

   Lentivirus for Dox-inducible expression of a non-targeting control shRNA

   Depositor

   Roland Friedel

   Insert

   non-targeting randomized sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   H1 / Tet-responsive element

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-MAP3K5 (WT)

   Plasmid

   #47104

   Purpose

   Expresses wild type human MAP3K5 in mammalian cells

   Depositor

   Yardena Samuels

   Insert

   MAP3K5 (MAP3K5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1-puro U6 sgRNA BfuAI large stuffer

   Plasmid

   #52628

   Purpose

   lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency

   Depositor

   Scot Wolfe

   Insert

   Kanamycin cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEMB44 / Mouse_LRRK2_1_342

   Plasmid

   #40555

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Lrrk2 (Lrrk2 Mouse)
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Mutation

   WT

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pEGFP-N3 alpha-SSeCKS

    Plasmid

    #61294

    Purpose

    Mammalian expression of GFP-alpha-SSeCKS

    Depositor

    Irwin Gelman

    Insert

    alpha SSeCKS (Akap12 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

11. human Alpha-synuclein

    Plasmid

    #51437

    Purpose

    human codon optimized Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pSecUAG_UTu1D

    Plasmid

    #233867

    Purpose

    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant

    Depositor

    Dieter Söll, Natalie Krahn

    Inserts

    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 32 switched to Selenocysteine (UAG) and …

    Promoter

    EM7, araBAD, and native As SelD

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

    Plasmid

    #39299

    Depositor

    Jennifer Tirnauer, Tim Mitchison

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR_SFFV*/Puro2a-GFP

    Plasmid

    #198222

    Purpose

    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP

    Depositor

    Eric Davis

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Codon-optimized puromycin resistance gene, follow…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSNAPf-H2B Control Plasmid

    Plasmid

    #101124

    Purpose

    Expression of SNAP-tagged Histone H2B in mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    histone cluster 1 H2B family member j (H2BC11 Human)
    

    Use

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pFLAG-CMV-hErk1

    Plasmid

    #49328

    Purpose

    Expresses FLAG-tagged human Erk1

    Depositor

    Melanie Cobb

    Insert

    Erk1 (MAPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-BE4max-P2A-EGFP (pRZ33)

    Plasmid

    #140083

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

    Depositor

    Keith Joung

    Insert

    Human codon optimized pCAG BE4max with P2A-EGFP
    

    Use

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A (SpCas9)

    Promoter

    CAG

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFLAG_G495R MDA5

    Plasmid

    #216799

    Purpose

    Expresses a single point mutant of melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G495R

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    constitutive 35SPPDK promoter

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX6P1-DEST

    Plasmid

    #119749

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid

    #196716

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-KRAB(ZIM3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Ad-FOXA2

    Plasmid

    #131581

    Purpose

    Expresses FOXA2 in mammalian cells

    Depositor

    Haiyan Xu

    Insert

    Forkhead box A2 (Foxa2 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    No

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCambia-loxKan

    Plasmid

    #199590

    Purpose

    basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility

    Depositor

    Andreas Stuermer

    Insert

    FRT-lox66-NosP-KanR-NosT-lox71-FRT
    

    Use

    Cre/Lox

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Nos

    Available since

    July 5, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pUC_cohNaV1.1_2009aa_IRES2_GFP

    Plasmid

    #227642

    Purpose

    Expresses Nav 1.1 and IRES-GFP

    Depositor

    Dravet Syndrome Foundation Spain

    Insert

    NaV1.1 (SCN1A Human)
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET50_WT MDA5

    Plasmid

    #216802

    Purpose

    Expresses wild type (wt) melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX6P1-N-HA

    Plasmid

    #119756

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQXCINeo-LOX

    Plasmid

    #134761

    Purpose

    a retroviral vector to express lysyl oxidase (derived from PCR4-TOPO-LOX)

    Depositor

    Martine Croset, Philippe Clezardin

    Insert

    LOX (LOX Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKAN43Ag8-GFP

    Plasmid

    #178182

    Purpose

    Plant binary vector expressing GFP by a enhanced 35S promoter

    Depositor

    Keiichi Shimizu

    Insert

    EGFP (eGFP )
    

    Use

    Tags

    Expression

    Plant

    Mutation

    The CAT is inserted after the start codon.

    Promoter

    The minimal 35S promoter from Cauliflower Mosaic …

    Available since

    Sept. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

    Plasmid

    #196971

    Purpose

    Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

    Depositor

    Leopold Parts

    Inserts

    Cas9-RT
    rtTA3G-P2A-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and TREG3G

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLVX-FLAG-mCherry-C1-centrin-1

    Plasmid

    #73333

    Purpose

    Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

    Depositor

    Manuel Thery

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG/HA-hBIG2

    Plasmid

    #226254

    Purpose

    Expresses N-terminally HA-tagged hBIG2 in mammalian cells

    Depositor

    Hye-Won Shin

    Insert

    BIG2 (ARFGEF2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid

    #50458

    Purpose

    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    rM3D-mCherry
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-hSyn-con/fon DREADD Gq-mCherry

    Plasmid

    #200661

    Purpose

    Construct for chemogenetically activating and visualizing subpopulations of neurons in the retina

    Depositor

    Samer Hattar

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51086

    Purpose

    Forebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa 1.3 kb

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUS250

    Plasmid

    #198322

    Purpose

    Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein

    Depositor

    Nicholas Coleman

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cumate-inducible

    Available since

    April 12, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pET23a_mod_HuD_FL

    Plasmid

    #65751

    Purpose

    plasmid for bacterial overexpression of HuD

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    6X His and FLAG HA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TAF1L

    Plasmid

    #39014

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TAF1L (TAF1L Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73039

    Purpose

    Express Nluc, copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin + CMV enhancer and chicken bet…

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. miniTurbo-dCasRx

    Plasmid

    #219821

    Purpose

    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and hU6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge