We narrowed to 10,941 results for: CHL

1. pCm N-term MBP NikJC199U

   Plasmid

   #174364

   Purpose

   Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, MBP, and TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCm N-term SMT3 NikJC199U

   Plasmid

   #174210

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, SMT3, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CDS12_TP-MpSIG2

   Plasmid

   #136093

   Purpose

   Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   TP-MpSIG2, Mapoly0214s0004
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. mt-DdCBE G1397-C

   Plasmid

   #174563

   Purpose

   mitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

   Depositor

   Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

   Insert

   DdCBE G1397-C
   

   Tags

   FLAG

   Expression

   Plant

   Promoter

   PcUbi

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mt-DdCBE G1397-N

   Plasmid

   #174562

   Purpose

   mitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

   Depositor

   Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

   Insert

   DdCBE G1397-N
   

   Tags

   HA

   Expression

   Plant

   Promoter

   PcUbi

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPRIChp-hOtub1

   Plasmid

   #174743

   Purpose

   Gene overexpression

   Depositor

   Shao-Cong Sun

   Article

   Zhou et al Nat Immunol. 2019 Jul;20(7):879-889. doi

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-EGFP-DmMe31B_268-459_D

   Plasmid

   #146119

   Purpose

   Insect Expression of DmMe31B_268-459

   Depositor

   Elisa Izaurralde

   Article

   Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

   Insert

   DmMe31B_268-459 (me31B Fly)
   

   Expression

   Insect

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGM9121_Cfo-CPO SP

   Plasmid

   #153500

   Purpose

   Caldariomyces (Leptoxyphium) fumago Chloroperoxidase Signal Peptide Donor Plasmid

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Insert

   Chloroperoxidase SP
   

   Use

   Synthetic Biology

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

   Plasmid

   #162811

   Purpose

   Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   BsmBI_(C1A)-Intein_DD
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   Intein(Cys1,Ala)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

    Plasmid

    #162815

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_M
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_M(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

    Plasmid

    #162816

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_9b
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_9b(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

    Plasmid

    #162817

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF6
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF6(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

    Plasmid

    #162818

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF7b
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF7b(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

    Plasmid

    #162819

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF10
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF10(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

    Plasmid

    #162820

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF9c/14
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF9c/14(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

    Plasmid

    #162821

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b_alt
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b_alt(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

    Plasmid

    #162822

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b(CoV1h)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b(CoV1h)(Met1,Amber)

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

    Plasmid

    #162805

    Purpose

    STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI-HA_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACYCDuet1-tglCDEF

    Plasmid

    #134677

    Purpose

    Expresses TglC, TglD, TglE, TglF in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglC
    TglD
    TglE
    TglF
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACYCDuet1-ammA(∆Trp56)

    Plasmid

    #134683

    Purpose

    Expresses His6-AmmA(∆Trp56) in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    ammA(delW56)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletion of W56

    Promoter

    T7

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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