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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,632 results for: t7

Showing: 2301 - 2320 of 10632 results

  1. TPH2

    Plasmid
    #73243
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TPH2 (TPH2 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73243-plasmid-map-sequence-id-137195

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  2. pmCellFree_KA1_SARS-COV-2_ORF3a

    Plasmid
    #169390
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169390-plasmid-map-sequence-id-336867

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  3. TBXTA-c043

    Plasmid
    #139766
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    Avi-tag (Biotin) and His6-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41- D225
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139766-plasmid-map-sequence-id-296581

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  4. pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)

    Plasmid
    #86933
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86933-plasmid-map-sequence-id-174748

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  5. HOXA5 (human) HIS-tag pET

    Plasmid
    #8562
    Depositor
    Corey Largman
    Insert
    HOXA5 (HOXA5 Human)
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    SacI site at amino acids 1-2 was used to clone in…
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  6. HARS_pNIC-Bio3

    Plasmid
    #153050
    Purpose
    Expression construct for producing Avi tagged histidyl tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    HARS (HARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153050-plasmid-map-sequence-id-304465

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  7. HOXC9 (murine) HIS-tag pET

    Plasmid
    #8559
    Depositor
    Corey Largman
    Insert
    HOXC9 (Hoxc9 Mouse)
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    makes protein by Western
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  8. SPIN2B (SPIN2BA-c011)

    Plasmid
    #98238
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPIN2BA (SPIN2B Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98238-plasmid-map-sequence-id-193417

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  9. pIVTR(T7A)-ATOH1 (SA Substitutions)

    Plasmid
    #172309
    Purpose
    In vitro transcription of ATOH1 (Ser phosphosites substituted by Ala)
    Depositor
    Patrik Verstreken
    Insert
    ATOH1 (ATOH1 Human)
    Use
    Other
    Mutation
    S331A, S342A
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172309-plasmid-map-sequence-id-340456

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  11. 4P7A

    Plasmid
    #61954
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. TBXTA-c027

    Plasmid
    #139764
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, G177D (TBXT Human)
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids S2- M435
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139764-plasmid-map-sequence-id-296577

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  14. CBS

    Plasmid
    #73238
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CBS (CBS Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    73238-plasmid-map-sequence-id-150431

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  15. pE-SUMO CRYBB3

    Plasmid
    #80750
    Purpose
    Expression of human beta B3-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBB3 (CRYBB3 Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80750-plasmid-map-sequence-id-152180

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  16. TBXTA-c044

    Plasmid
    #139767
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    Avi-tag (Biotin) and His6-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41- D225
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139767-plasmid-map-sequence-id-296582

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  17. 5E16 (PfPKG, PF14_0346)

    Plasmid
    #79518
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0346
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79518-plasmid-map-sequence-id-150101

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  18. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available Since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

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  19. WNK1 (WNK1A-c011)

    Plasmid
    #98232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    WNK1A (WNK1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98232-plasmid-map-sequence-id-193412

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  20. TP53BP1

    Plasmid
    #73252
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TP53BP1A (TP53BP1 Human)
    Tags
    His6-Thrombin
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73252-plasmid-map-sequence-id-136217

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Showing: 2301 - 2320 of 10632 results