We narrowed to 2,382 results for: T2A

1. Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

   Plasmid

   #231907

   Purpose

   For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   EGFP-P2A-dTomato-T2A-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

   Plasmid

   #227009

   Purpose

   Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

   Plasmid

   #227010

   Purpose

   Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

   Plasmid

   #227011

   Purpose

   Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

   Plasmid

   #227012

   Purpose

   Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

   Plasmid

   #225588

   Purpose

   Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2(S54L+T127A)-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry

   Plasmid

   #225584

   Purpose

   Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2 (S54L+T127A)-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T21R-F8.2 (S54L+T127A)

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

   Plasmid

   #227773

   Purpose

   Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Inserts

   Cas9-t2A-GFP
   4xU6:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 and tp1

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

   Plasmid

   #221447

   Purpose

   Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   SPR degron: hGEM (1-110) fragment that is fused t…

   Promoter

   CMV

   Available since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pTY186 EGFP-T2A-ALFA-ORF6 SARS-CoV-2

    Plasmid

    #204976

    Purpose

    Co-express EGFP and SARS-CoV-2 ORF6 N-terminally labeled with ALFA tag

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    ALFA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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