We narrowed to 16,804 results for: URE

1. pDEST-mCherry-LacR-BRCA1

   Plasmid

   #71115

   Purpose

   mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP2135

   Plasmid

   #72248

   Purpose

   Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, and D1135E

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

   Plasmid

   #157974

   Purpose

   Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

   Depositor

   Carolyn McBride

   Article

   Zhao et al Nature. 2022 May;605(7911):706-712. doi:

   Insert

   Orco
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX neo PFKP-GFP K281R

   Plasmid

   #138288

   Purpose

   Mammalian Expression, Lentiviral. Expression of human phosphofructokinase platelet isoform PFKP-GFP fusion protein with K281R mutation

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   PFKP (PFKP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K281R

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

   Plasmid

   #47458

   Purpose

   LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBF_HsPepT1

   Plasmid

   #73943

   Purpose

   Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

   Depositor

   Simon Newstead

   Article

   Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

   Insert

   PepT1 (SLC15A1 Human)
   

   Use

   Make rna for xenopus oocyte injection

   Tags

   Flag

   Expression

   Mutation

   Promoter

   beta globin

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pGEX6P1-HA-MEK5

   Plasmid

   #53166

   Purpose

   Expresses GST-HA tagged human MEK5 from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK5 (MAP2K5 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBF_HsPepT2

   Plasmid

   #73944

   Purpose

   Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

   Depositor

   Simon Newstead

   Article

   Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

   Insert

   PepT2 (SLC15A2 Human)
   

   Use

   Make rna for xenopus oocyte injection

   Tags

   Flag

   Expression

   Mutation

   Promoter

   beta globin

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

9. BPK2797

   Plasmid

   #72251

   Purpose

   Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, and T1337R

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47455

    Purpose

    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    

    Use

    AAV

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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