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pIG-705pUC19-tNGFR-P2A-STAT5b

Plasmid

#186103

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

STAT5B (STAT5B Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-460 tNGFR-P2A-STAT4

Plasmid

#186075

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

STAT4 (STAT4 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-455 tNGFR-P2A-WAS

Plasmid

#186082

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

WAS (WAS Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS10-riboE-dddAI

Plasmid

#186561

Purpose

Regulated expression of the DddA immunity determinant (DddAI) in Firmicutes

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Use

Tags

Expression

Bacterial

Mutation

Promoter

pSpac(hy)

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-HDAC5-trun1)

Plasmid

#114400

Purpose

For PYL-HDAC5 aa 1-275 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 aa 1-275 (HDAC5 Human)

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pIE-N-hα2M-H6

Plasmid

#128493

Purpose

Expresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (S24-E908) (A2M Human)

Use

Tags

His tag (6x)

Expression

Insect

Mutation

Promoter

IE1

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pS6-N-hα2M-H6

Plasmid

#128496

Purpose

Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (G27-A788) (A2M Human)

Use

Tags

His tag (6x)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pIG-641 JE-TRAC-BCMA(BB)28z-P2A

Plasmid

#186127

Purpose

BCMA-CAR DNA production

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

BCMA-CAR (TNFRSF17 Human)

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAB(EXPR-PYL-HDAC3-NEO)

Plasmid

#114394

Purpose

For PYL-HDAC3 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC3 (HDAC3 Human)

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIE-TGFB2-H6

Plasmid

#128500

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Use

Tags

His tag (6x)

Expression

Insect

Mutation

Promoter

IE1

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pS6-TGFB2-H8

Plasmid

#128502

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (L21-S414) (TGFB2 Human)

Use

Tags

His tag (6x)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

Plasmid

#68345

Purpose

A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

Depositor

Jannik Elverløv-Jakobsen

Article

Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

Inserts

puromycin
hCas9

Use

CRISPR; Flp/frt

Tags

linked to red flouresent protein gene mKateII and…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-dddAI-FLAG

Plasmid

#186560

Purpose

Broad host range regulated expression of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Use

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4-His-Max-A-PQBP1-Y65C

Plasmid

#25803

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Apr 21. ():.

Insert

PQBP1 with Y65C mutation (PQBP1 Human)

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

Tyrosine 65 within the WW domain of PQBP1 is chan…

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pS6-TGFB2-STREP

Plasmid

#128503

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Use

Tags

STREP (2X) tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pIE-C-hα2M-H6

Plasmid

#128494

Purpose

Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (R787-N1473) (A2M Human)

Use

Tags

His tag (6x)

Expression

Insect

Mutation

contains R787-N1473

Promoter

IE1

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSV39-dddAI-FLAG

Plasmid

#186558

Purpose

Broad host range regulated expression of the DddA with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Use

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS10-ribE-dddAI-FLAG

Plasmid

#186562

Purpose

Regulated expression in Firmicutes of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Use

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

pSpac(hy)

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL4-DBD-EGR1-WT

Plasmid

#215592

Purpose

For overexpression of Gal4-DBD EGR1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 (EGR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroSCRAMBLED

Plasmid

#215594

Purpose

For overexpression of Gal4-DBD EGR1 AroSCRAMBLED

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroSCRAMBLED (EGR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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