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pFA6a-mCherry-hphMX6

Plasmid

#105156

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

mCherry-hphMX6

Tags

mCherry

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pICE-HA-NLS-I-PpoI

Plasmid

#46963

Purpose

For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

NLS-I-PpoI

Tags

HA

Expression

Mammalian

Promoter

CMV-TET

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs ABCDE>A

Plasmid

#83318

Purpose

human DNA-PKcs/ABCDE>ala

Depositor

Katheryn Meek

Article

Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

6 phosphorylation sites in the ABCDE cluster subs…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Lyso-RA

Plasmid

#209875

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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FHRE-Luc

Plasmid

#1789

Purpose

Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FHRE (FOXO3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

3 copies of FRE

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct5

Plasmid

#28198

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa257-414 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
TagBFP2-CAAX

Plasmid

#116856

Purpose

plasma membrane marker

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

HRAS

Tags

TagBFP2

Expression

Mammalian

Mutation

Amino acids 172-189

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP(HiBiT)

Plasmid

#162589

Purpose

N-terminally HiBiT-tagged EGFP under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

EGFP

Tags

HiBiT

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET22b_PstS_3

Plasmid

#78200

Purpose

Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

Depositor

Martin Webb

Article

Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

Insert

pstS (A17C,I76G,A197C)

Expression

Bacterial

Mutation

A17C,I76G,A197C (please see depositor comment bel…

Promoter

T7

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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TK4_CLYBL_targeting

Plasmid

#254455

Purpose

TK4 targeting vector containing left and right homology arms for the CLYBL locus

Depositor

Marius Wernig

Article

Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2

Inserts

mycNLS-mScarlet-T2A-mNeonGreen-PEST
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS

Expression

Mammalian

Promoter

CAG and EF1a

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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