We narrowed to 3,711 results for: mak

1. pSaCas9-1xPP7-2x3'UTR

   Plasmid

   #122947

   Purpose

   For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR

   Depositor

   Baisong Lu

   Article

   Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

   Insert

   SaCas9-PP7-HBB 3' UTR
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn Con/Fon EYFP

   Plasmid

   #55650

   Purpose

   Cre-on/Flp-on EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Insert

   eYFP with introns
   

   Use

   AAV; Cre on/flp on eyfp

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-AmCyan

   Plasmid

   #138481

   Purpose

   For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   AmCyan
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-Tet-On-ARNTL-shRNA1

   Plasmid

   #166913

   Purpose

   Lentivirus for inducible knockdown of human ARNTL(BMAL1)

   Depositor

   Roland Friedel

   Article

   Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

   Insert

   ARNTL shRNA (BMAL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

   Plasmid

   #55643

   Purpose

   vCre-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV; Vcre-dependent chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-HA-FbxO11-pcDNA3.1-

   Plasmid

   #52501

   Purpose

   expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO11 (FBXO11 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MBP-FUS_LC_WT

   Plasmid

   #98653

   Purpose

   Expresses MBP-tagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-ChRmine-mScarlet

   Plasmid

   #137158

   Purpose

   Viral expression of ChRmine-p2a-mScarlet

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   ChRmine-p2a-mScarlet
   

   Use

   AAV

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-HA-FbxO5-pcDNA3.1-

   Plasmid

   #52509

   Purpose

   expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO5 (FBXO5 Human, FbxO5)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT Smarca4-V5 IRES-puro

    Plasmid

    #107058

    Purpose

    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

    Depositor

    Jerry Crabtree, Courtney Hodges

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-puroR and V5

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CAG

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. mIFP-TOMM20-N-10

    Plasmid

    #56239

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    NM_014765.2 (TOMM20 Human)
    

    Tags

    mIFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pAAV-hSyn Coff/FonEYFP

    Plasmid

    #55652

    Purpose

    Cre-off/Flp-on EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    eYFP with introns
    

    Use

    AAV; Cre off/flp on eyfp

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. MBP-FUS_FL_12E

    Plasmid

    #98652

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    12 S/T to E

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Luciferase

    Plasmid

    #213979

    Purpose

    Expresses Doxy Inducible Luciferase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    6xHis affinity tag

    Expression

    Bacterial and Mammalian

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas-dCas12m

    Plasmid

    #192275

    Purpose

    Encodes MmdCas12m (D485A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInt-ERCreER

    Plasmid

    #190040

    Purpose

    This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

    Depositor

    Kayoko Tanaka

    Article

    Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

    Insert

    ERT2-Cre-ERT2 flanked by AAVS1
    

    Use

    CRISPR and TALEN

    Expression

    Mammalian

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 8xNFAT-ZsG-muhCD4

    Plasmid

    #162745

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Human CD4 gene with glutamine to tyrosine at posi…

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas-Cas12m

    Plasmid

    #192274

    Purpose

    Encodes MmCas12m under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO-Tet-On-ARNTL-shRNA2

    Plasmid

    #166914

    Purpose

    Lentivirus for inducible knockdown of human ARNTL(BMAL1)

    Depositor

    Roland Friedel

    Article

    Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

    Insert

    ARNTL shRNA (BMAL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRXIP-GFP-CERT(PHD)

    Plasmid

    #221746

    Purpose

    Stable expression of GFP-CERT(PHD)in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    CERT (CERT1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–116

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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