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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,354 results for: 11

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Showing: 2341 - 2360 of 8354 results

  1. pcDNA3-PM-ExRai-CKAR2

    Plasmid
    #236098
    Purpose
    Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.
    Depositor
    Jin Zhang
    Publication
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-ExRai-CKAR2
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236098-plasmid-map-sequence-id-473738

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  2. pJMP3664

    Plasmid
    #222350
    Purpose
    Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Publication
    Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222350-plasmid-map-sequence-id-439070

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  3. pC0091

    Plasmid
    #181955
    Purpose
    Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    7xFOP
    Expression
    Mammalian
    Available since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    181955-plasmid-map-sequence-id-354480

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  6. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid
    #71829
    Purpose
    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BACH2-sgRNA8 (BACH2 Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71829-plasmid-map-sequence-id-131024

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  7. R57C10-Flp2

    Plasmid
    #63172
    Purpose
    R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion
    Depositor
    Gerald Rubin
    Publication
    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p
    Insert
    yeast wild type containing a Glycine at amino acid 5 of the protein sequence
    Expression
    Insect
    Available since
    July 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pcDNA-NES-ZapCmR2

    Plasmid
    #59015
    Purpose
    Cytosolic zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    Tags
    Nuclear Exclusion Signal
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. 645 pCS2-etv2

    Plasmid
    #49005
    Purpose
    Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2
    Depositor
    Nathan Lawson
    Publication
    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ets variant gene 2 (etsrp Zebrafish)
    Use
    Invitro transcription, riboprobe template
    Expression
    Mammalian
    Available since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    49005-plasmid-map-sequence-id-71928

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  13. KRAB-dSpCas9

    Plasmid
    #205248
    Purpose
    In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.
    Depositor
    Rasmus Bak
    Publication
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    KRAB-dSpCas9
    Use
    CRISPR
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205248-plasmid-map-sequence-id-409108

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  15. pTol2-CreLite

    Plasmid
    #131783
    Purpose
    Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.
    Depositor
    George Eisenhoffer
    Publication
    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PhyBCreC, PIF6CreN, and mTagBFP2
    Use
    Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…
    Tags
    mTagBFP2
    Expression
    Mammalian
    Promoter
    Ubi
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    131783-plasmid-map-sequence-id-268078

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  16. STARR-seq luciferase validation vector_ORI_SV40

    Plasmid
    #99309
    Purpose
    Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Publication
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV40 enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available since
    Sept. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    99309-plasmid-map-sequence-id-190937

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Showing: 2341 - 2360 of 8354 results