We narrowed to 2,370 results for: mia

1. pET28b-Ets1-ETS

   Plasmid

   #85735

   Purpose

   Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440

   Depositor

   Gregory Poon

   Article

   Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

   Insert

   DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Human, Mouse)
   

   Use

   Tags

   6xHis following thrombin cleavage site LVPR|G

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG8Ha-FLI1_DBD_R2L2

   Plasmid

   #188049

   Purpose

   Expresses His8-FLI1_DBD_R2L2(276-399_R337L_R340L_F362A) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-275, 400-452, mutated R337L…

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG8Ha-FLI1_DBD_deltaAlpha4

   Plasmid

   #188050

   Purpose

   Expresses His8-FLI1_DBD_deltaAlpha4(276-361) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-275, 362-452

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG8Ha-FLI1_DBD(276-399_F362A)

   Plasmid

   #188047

   Purpose

   Expresses His8-FLI1_DBD(276-399_F362A) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

   Insert

   FLI1 (FLI1 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-275, 400-452, mutated F362A

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. MoFGFR31c-ires neo

   Plasmid

   #86496

   Purpose

   mamalian cell expression of FGFR3-FGFR1 chimera

   Depositor

   David Ornitz

   Article

   Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

   Insert

   FgfrR3-1 IIIc 15C7 (Fgfr1, Fgfr3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FGFR3-FGFR1 chimera

   Promoter

   Moloney murine leukemia virus LTR

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MoFGFR31b-ires neo

   Plasmid

   #86495

   Purpose

   mamalian cell expression of FGFR3-FGFR1 chimera

   Depositor

   David Ornitz

   Article

   Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

   Insert

   FgfrR31 IIIb 15C8 (Fgfr3, Fgfr1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FGFR3-FGFR1 chimera

   Promoter

   Moloney murine leukemia virus LTR

   Available since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PEmax-P2A-hMLH1dn

   Plasmid

   #174828

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLXSN p200 CUX1

   Plasmid

   #90470

   Purpose

   Retroviral vector expressing human p200 CUX1 with a Myc and HA tag at the N- and C-terminus, respectively

   Depositor

   Alain Nepveu

   Article

   Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

   Insert

   CUX1 (CUX1 Human)
   

   Use

   Retroviral

   Tags

   Ha and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Moloney murine leukemia virus long terminal repeat

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PE7-P2A-hMLH1dn

   Plasmid

   #222997

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Deletion of residues 754-756 in MLH1 as described…

   Promoter

   CMV

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Use

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-UBR5 ∆HECT

    Plasmid

    #52051

    Purpose

    CMV driven mammalian expression of ligase-dead UBR5 with an N-termianl GFP fusion

    Depositor

    Darren Saunders

    Article

    Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

    Insert

    UBR5 ∆HECT (UBR5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C2769A

    Promoter

    CMV

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PE2-P2A-hMLH1dn

    Plasmid

    #174827

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-hMLH1dn
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PEA1-Puro-HEK3 CTT ins

    Plasmid

    #171996

    Purpose

    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDN-D2irTNG4kwh

    Plasmid

    #44722

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    htetR::NLS::EGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter;…

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PEmax-P2A-hP53DD

    Plasmid

    #214084

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PEmax-P2A-hP53DD (TP53 Synthetic, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PEA1-Nuclease-Puro HEK3 CTT ins

    Plasmid

    #171995

    Purpose

    Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG8Ha-FLI1_DBD(276-399_C299S_F362A_S390C)

    Plasmid

    #188048

    Purpose

    Expresses His8-FLI1_DBD(276-399_C299S_F362A_S390C) with a TEV site for removal of the His8 tag.

    Depositor

    David Libich

    Article

    Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-275, 400-452, mutated C299S…

    Promoter

    T7

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

    Plasmid

    #236241

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV1.2 (CACNA1C Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

    Plasmid

    #236239

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV2.1 (CACNA1A Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

    Plasmid

    #236240

    Purpose

    Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    CaV2.2 (CACNA1B Rabbit)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge