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pReporter_ZF1_mNeonGreen

Plasmid

#213840

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-1

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen

Plasmid

#213841

Purpose

Facilitates genomic integration of the reporter with upstream binding sites for ZF-2

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pStorage_Ptet-1_ZF1

Plasmid

#213844

Purpose

CombiGEM storage plasmid for ZF-1 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pStorage_Ptet-2_ZF2

Plasmid

#213845

Purpose

CombiGEM storage plasmid for ZF-2 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLEU

Plasmid

#213846

Purpose

LEU integrating plasmid backbone used to create all of the reporter plasmids.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_TetR

Plasmid

#213847

Purpose

Facilitates genomic integration of tetR expression cassette.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

TetR

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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His-MBP-FL-Efg1

Plasmid

#216395

Purpose

Expresses MBP-tagged full length Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-FL

Tags

6xHis tags-MBP

Expression

Bacterial

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-C-PrLD

Plasmid

#216398

Purpose

Expresses 6xHis-tagged C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-C-PrLD

Tags

6xHis-tags

Expression

Bacterial

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-DBD

Plasmid

#216399

Purpose

Expresses 6xHis-tagged DBD of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-DBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 2-181 and 357-554

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pActin-miRFP704nano

Plasmid

#206191

Purpose

Human beta-actin labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano-human beta-actin

Tags

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pClathrin-miRFP704nano

Plasmid

#206193

Purpose

Clathrin labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano-clathrin light chain

Tags

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMyosin-miRFP704nano

Plasmid

#206194

Purpose

LC-Myosin labelled with near-infrared fluorescent protein miRFP704nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

Insert

miRFP704nano-LC-Myosin

Tags

miRFP704nano

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh2-2300

Plasmid

#170984

Purpose

Encodes sgRNA target 2300 and Kanamycin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh3-2300

Plasmid

#170985

Purpose

Encodes sgRNA target 2300 and Tetracycline resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh8-2300

Plasmid

#170986

Purpose

Encodes sgRNA target 2300 and Hygromycin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRO16Sh5

Plasmid

#180076

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLac

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC6

Plasmid

#198429

Purpose

Cloning of promoter of interest in front of mkate2 reporter gene

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-CtDCP1_X

Plasmid

#148006

Purpose

Bacterial Expression of CtDCP1

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP1

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C-C-term_F

Plasmid

#146306

Purpose

Mammalian Expression of HsTNRC6C-Cterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6C-Cterm (TNRC6C Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO4_F

Plasmid

#146317

Purpose

Mammalian Expression of HsAGO4

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO4 (AGO4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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