We narrowed to 6,934 results for: crispr cas9 plasmids

1. DSN1 C11.1 gRNA

   Plasmid

   #90656

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DSN1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DSN1 (Guide Designation C11.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. DSN1 C12.1 gRNA

   Plasmid

   #90657

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DSN1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DSN1 (Guide Designation C12.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CENPF C6.1 gRNA

   Plasmid

   #90617

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CENPF

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CENPF (Guide Designation C6.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ESPL1 D7.1 gRNA

   Plasmid

   #90682

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ESPL1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   ESPL1 (Guide Designation D7.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GINS1 D11.1 gRNA

   Plasmid

   #90694

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human GINS1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   GINS1 (Guide Designation D11.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-sg-mGreenLantern/EGFP

   Plasmid

   #179913

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern/EGFP sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-sg-tdTomato90/816

   Plasmid

   #179918

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX459-sg-tdTomato166/892

   Plasmid

   #179919

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBtet-RB-PE2

   Plasmid

   #173903

   Purpose

   SB-transposon with inducible expression of SpCas9 PE2

   Depositor

   Ronald Cohn

   Insert

   PE2
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBbi-RB-PE2

    Plasmid

    #173902

    Purpose

    SB-transposon with constitutive expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FASTHDR-Cterm-mClover3-Puromycin

    Plasmid

    #167205

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FASTHDR-Cterm-HaloTag-Puromycin

    Plasmid

    #167208

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FASTHDR-Cterm-mRuby3-Zeo

    Plasmid

    #167206

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FASTHDR-Cterm-mTagBFP2-Blasticidin

    Plasmid

    #167207

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FASTHDR-Cterm-NanoLuc-Puromycin

    Plasmid

    #167209

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC57-Simple-gRNA backbone

    Plasmid

    #51306

    Purpose

    gRNA expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Type

    Empty backbone

    Use

    CRISPR; Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid

    #68357

    Purpose

    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and synthetic Probasin ARRx2

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNR4A1.1.0-gDNA

    Plasmid

    #112391

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1

    Depositor

    Kevin White

    Insert

    NR4A1 (NR4A1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTEAD1.1.0-gDNA

    Plasmid

    #112425

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TEAD1

    Depositor

    Kevin White

    Insert

    TEAD1 (TEAD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTFAP2B.1.0-gDNA

    Plasmid

    #113791

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2B

    Depositor

    Kevin White

    Insert

    TFAP2B (TFAP2B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge