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Plasmid#139595Purposeexpresses KinL in E. coliDepositorInsertkinL
UseTagshexahistidineExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KinM
Plasmid#139594Purposeexpresses KinM in E. coliDepositorInsertkinM
UseTagshexahistidineExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KinN
Plasmid#139593Purposeexpresses KinN in E. coliDepositorInsertkinN
UseTagshexahistidineExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-MacroGreen
Plasmid#160665PurposeExpresses GFP fusion of a multisite mutant of the Af1521 proteinDepositorInsertAf1521 modified synthetic construct eGFP fusion
UseTagseGFP and hexahistidine_TEV recognitionExpressionBacterialMutationPromoterLacIAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
bZiPro
Plasmid#86846Purposeexpress NS2B-NS3 protease in E.coliDepositorInsertsNS3 1-177
NS2B45-96
UseTagsa hexahistidine tag and a thrombin cleavage siteExpressionBacterialMutationNS2B cofactor region (residues 45–96) and NS3 pro…PromoterT7Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR hH1-TERRA
Plasmid#111381PurposeTERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminatorDepositorInserthH1-TERRA
UseGateway entry cloningTagsExpressionMutationPromoterhH1Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-YpODH
Plasmid#110806PurposeExpresses YpODH and opine dehydrogenase from Yersinia pestsDepositorInsertYPO1347 (YPO1347 )
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBz118_His_CsoS2
Plasmid#140841PurposeHalothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backboneDepositorInsertCsoS2
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz54_polyPro_polar
Plasmid#140847PurposeSame as pLz47 but with 20 polar amino acids instead of N*- peptideDepositorInsertpolyproline helix with polar residues
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz58_polyPro_CsoS2_CTP
Plasmid#140850PurposeSame as pLz47 but with C-terminal peptide (CTP) instead of N*- peptideDepositorInsertpolyproline helix with CsoS2 C-terminal peptide
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz55_polyPro_random_Npeptide
Plasmid#140846PurposeSame as pLz47 but with randomized N*-peptide sequenceDepositorInsertpolyproline helix with randomized CsoS2 N-peptide
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz56_polyPro_CsoS2_MR_r1
Plasmid#140848PurposeSame as pLz47 but with MR repeat 1 instead of N*-peptideDepositorInsertpolyproline helix with CsoS2 MR repeat 1
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz57_polyPro_CsoS2_CTD_r8
Plasmid#140849PurposeSame as pLz47 but with CTD repeat 8 instead of N*-peptideDepositorInsertpolyproline helix with CsoS2 CTD repeat 8
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
2QO2 (EPHA3)
Plasmid#101271PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEPHA3 (EPHA3 Human)
UseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBP-HexHis
Plasmid#72983PurposeHexahistidine N-terminal tag (5' TAAA/3' CATA fusion)DepositorInsertPolyhistidine (HHHHHH) N-terminal tag
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q)-6xHis
Plasmid#176576PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
HTATIP (2OU2)
Plasmid#33338PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP (KAT5 Human)
UseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29b-dspB(E184Q)-6xHis
Plasmid#175802PurposePlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tagExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRCHisB-TRF2∆B
Plasmid#53208PurposeExpressed N-terminally Hexahistine tagged TRF2 Delta Basic proteinDepositorInsertTRF2 Delta Basic (TERF2 Human)
UseTags6x HisExpressionBacterialMutationcontains amino acids 47-500PromoterTrcAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP7
Plasmid#71470PurposeEncodes human full length SENP7. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 7 (SENP7 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only