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pEF1-FRT-HaloTag-TAF1A

Plasmid

#247347

Purpose

Expresses HaloTag-TAF1A

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1A (TAF1A) (TAF1A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-RnCENP-W

Plasmid

#238064

Purpose

Expresses rat CENP-W; untagged; made for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Rat CENP-W (Cenpw Rat)

Expression

Mammalian

Promoter

T7

Available Since

May 29, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 (N)ZF1-3 (1-173)

Plasmid

#235646

Purpose

Bacterial expression of human CAPN15 (N)ZF1-3 (1-173)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 (N)ZF1-3 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 1-173 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 NZF2 (34-72)

Plasmid

#235645

Purpose

Bacterial expression of human CAPN15 NZF2 (34-72)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 NZF2 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 34-72 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 NZF1 (1-42)

Plasmid

#235644

Purpose

Bacterial expression of human CAPN15 NZF1 (1-42)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 NZF1 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 1-42 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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Reckleen_3plus_sgRNA_ptet_pos5a

Plasmid

#233461

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a1

Plasmid

#233462

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5a2

Plasmid

#233463

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b

Plasmid

#233464

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b1

Plasmid

#233465

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Reckleen_3plus_sgRNA_ptet_pos5b2

Plasmid

#233466

Purpose

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, for building RECKLEEN plasmid with multiple sgRNAs

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, ColE1 ori, and KanR.

Use

CRISPR

Available Since

April 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-Twin-strep-TEV-DGKδ2

Plasmid

#223720

Purpose

Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

Depositor

Chiaki Murakami

Insert

DGKD (DGKD Human)

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

deleted start codon

Promoter

CAG and chicken β-actin promoter

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-PD-L1

Plasmid

#223571

Purpose

Lentiviral expression of human PD-L1 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A-GS3

Plasmid

#204753

Purpose

Expression of Cas9 and human H2A

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2A (H2AC20 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-shWT1_A

Plasmid

#217636

Purpose

Dox inducible shRNA targeting WT-1

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shWT1_A (WT1 Human)

Use

RNAi

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_E192K_D193K

Plasmid

#214322

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

E192K_D193K

Promoter

T7

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_I48N

Plasmid

#214323

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

I48N

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_mouse-Pro-IL-18_humanized-triple-mutant

Plasmid

#214339

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (Il18 Mouse)

Tags

His-TEV

Expression

Bacterial

Mutation

ENGD29DDEN_N36Y_H41E

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_LES33AAA

Plasmid

#214326

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

LES33AAA

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_DDEN29ENGD

Plasmid

#214325

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

DDEN29ENGD

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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