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pONL-N1_TUBB5

Plasmid

#65680

Purpose

Expresses beta-tubulin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_OCT4_T234A_S235A

Plasmid

#40634

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Mammalian

Mutation

T234A_S235A

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
p6619 MSCV-IP N-HAonly UBE3AI

Plasmid

#35015

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

UBE3AI

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pBacPAK8-myc-Rbx1

Plasmid

#29507

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Rbx1 (Rbx1 Mouse)

Tags

myc

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pEBB-GFP-hBEN-deltaNLS3

Plasmid

#22158

Depositor

Ananda Roy

Article

Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

Insert

hBEN (GTF2IRD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 883-889

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
myc-EAF pBacPAK8

Plasmid

#15324

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor (EAF1 Human)

Use

Baculovirus construction

Tags

Myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
3222 pSFFV-neo Bad S136A

Plasmid

#8759

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

S136A

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pA_higher-Opto_N449S

Plasmid

#253258

Purpose

Opto-CheY N449S expressed using the proA promoter and wild type T7 RBS

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

wild type T7 RBS

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_N449S

Plasmid

#253256

Purpose

Opto-CheY N449S expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with N449S mutation

Tags

T7 RBS variant GAAGGAGgT

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pA-Opto_V416T

Plasmid

#253257

Purpose

Opto-CheY V416T expressed using the proA promoter and T7 RBS variant GAAGGAGgT

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

Opto-CheY with V416T mutation

Expression

Bacterial

Mutation

T7 RBS variant GAAGGAGgT

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.S29W.A95K.mRuby3

Plasmid

#244911

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mRuby3

Use

AAV

Mutation

S29W.A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95A.A119L.mIRFP670nano3

Plasmid

#244917

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mIRFP670nano3

Use

AAV

Mutation

A95A.A119L

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mIRFP670nano3

Plasmid

#244916

Purpose

iATPSnFR2.A95K variant with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mIRFP670nano3

Use

AAV

Mutation

A95K

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(T42A)

Plasmid

#245223

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

T42A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(E76K)

Plasmid

#245227

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

E76K

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/T119M

Plasmid

#242712

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, T119M

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/V122I

Plasmid

#238122

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, V122I

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/F87A

Plasmid

#238120

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, F87A

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/V30M

Plasmid

#238119

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, V30M

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mRuby3

Plasmid

#244912

Purpose

iATPSnFR2.A95K variant with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mRuby3

Use

AAV

Mutation

A95K

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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