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pcDNA3-YFP-PKD2

Plasmid

#31821

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Insert

YFP-Protein Kinase D2 (PRKD2 Human)

Tags

YFP

Expression

Mammalian

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-MST1R

Plasmid

#23942

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MST1R (MST1R Human)

Use

Gateway Cloning

Mutation

R322Q

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-Myc tag

Plasmid

#67617

Purpose

A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Myc

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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F-Cdk8

Plasmid

#15366

Depositor

Joan Conaway, Ronald Conaway

Article

Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

Insert

CDK8 (CDK8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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hFLT1

Plasmid

#83435

Purpose

human VEGFR1 with HA and FLAG tag

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

Insert

human VEGFR1 (FLT1 Human)

Tags

Flag and Myc

Expression

Mammalian

Mutation

HA-tag was inserted 30 AA downstream of the FLT1 …

Promoter

CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP2-Flag-His-WT

Plasmid

#111574

Purpose

Expresses full length PARP2 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP2 (PARP2 Human)

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Promoter

polyhedrin promoter

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDB6 (CK2alpha')

Plasmid

#27084

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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Myr.PKCzeta.FLAG

Plasmid

#10802

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG and Myr

Expression

Mammalian

Available Since

July 26, 2006

Availability

Academic Institutions and Nonprofits only
pCasKP-hph

Plasmid

#117232

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB555

Plasmid

#74885

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB404

Plasmid

#74798

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pORTMAGE311B

Plasmid

#120418

Purpose

Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

Depositor

Csaba Pál

Article

Szili et al Antimicrob Agents Chemother. 2019 Aug 23

Inserts

MutL E32K
Lambda Beta
XylS

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Promoter

Pm

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR1-Fv-Fvls-E

Plasmid

#15285

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FGFR1 cytoplasmic domain FKBP12 F36V

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag HK1

Plasmid

#20612

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HK1 (HK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
VN-alpha Synuclein

Plasmid

#89470

Purpose

expresses human alpha Synuclein fused to the N-terminal part of Venus

Depositor

Bradley Hyman, Tiago Outeiro

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Rheb (Q64L)-pcw107

Plasmid

#64607

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Mutation

Q64L

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
hRIP3 GFP D160N

Plasmid

#41386

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D160N)

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP

Plasmid

#32139

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3RT> STOP STOP B3RT>

Expression

Insect

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223-LYN

Plasmid

#23905

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LYN (LYN Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only