-
Plasmid#195982PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Y276H PHYB-CAAX
Plasmid#154911Purpose1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.DepositorInsertPhytochrome B (PHYB Mustard Weed)
UseXenopus/avian/zebrafishTagsCAAX membrane moiety and mCherryExpressionMammalianMutation1-621 amino acids of Arabidopsis PHYB, Tyrosine r…PromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha A-crystallin 1kb promoter/AcGFP
Plasmid#98096PurposeaA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha A-crystallin 1 kb fragment (-1028/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
791 p3E-2A-pCS2MCSpA v2
Plasmid#195973PurposeGateway p3E 2A fluorophoreDepositorInsert2A-multiple cloning site
UseOtherTagsExpressionMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-CDC42
Plasmid#109531PurposeMuscle specific zebrafish expression of mKate2 fused to CDC42DepositorInsertCDC42 (cdc42 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
ACVR1-TAAS-R206H
Plasmid#206184Purposein vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206HDepositorInsertactivin A receptor type 1 (ACVR1 Human)
UseIn vitro transcriptionTagsFLAG tagExpressionMutationS190A, S192A, R206HPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-CDC42-DN
Plasmid#109532PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative CDC42DepositorInsertCDC42 (cdc42 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationDNPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG657 H2B-pFAST
Plasmid#172862PurposeExpresses pFAST fused to zebrafish histone H2B in mammalian cellsDepositorInsertH2B-pFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-I152L-GGGS
Plasmid#162613PurposeAllows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmVenus
UseTagsExpressionMammalianMutation1-155-I152LPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119067PurposeDonor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertNLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119065PurposeDonor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertHA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a-EGFP
Plasmid#205311PurposeExpression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a-EGFP
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a
Plasmid#205312PurposeExpression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mKate2
Plasmid#162624PurposeAllows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assaysDepositorInsertmKate2
UseTagsExpressionMammalianMutationPromoterSP6Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FnoCas12a-2C-NLS in pCSDest
Plasmid#126639PurposeExpresses FnoCas12a-2C-NLS in mammalian cellsDepositorInsertFnoCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK036 desmaMCS:minCerulean,cryaa:Venus
Plasmid#195981PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, cryaa Venus (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -