Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,457 results for: golden gate

Clear keywords
Showing: 2401 - 2420 of 5457 results

  2. Tad-CBE(TCA3)

    Plasmid
    #235064
    Purpose
    Expresses Tad-CBE(TCA3) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(TCA3)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235064-plasmid-map-sequence-id-466802

    Enlarge

  9. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

    Plasmid
    #228663
    Purpose
    Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Zika virus portion of NS2B protease coexpressed with NS3 protease
    Tags
    His-GST-TEV
    Expression
    Bacterial
    Available since
    Feb. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    228663-plasmid-map-sequence-id-461604

    Enlarge

  10. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227890
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227890-plasmid-map-sequence-id-455730

    Enlarge

  12. CROPseq-Puro-24xMS2

    Plasmid
    #212629
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Publication
    Najia et al Cell. 2026 Sep 1:S0092-8674(26)00932-3.
    Insert
    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    212629-plasmid-map-sequence-id-420222

    Enlarge

  18. pHEE-R-hyg9si

    Plasmid
    #222832
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222832-plasmid-map-sequence-id-446482

    Enlarge

Showing: 2401 - 2420 of 5457 results