We narrowed to 5,211 results for: golden gate
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Plasmid#235074PurposeExpresses Tad-CBE(CCG3) in mammalian cells.DepositorInsertTad-CBE(CCG3)-SpCas9 D10A
UseCRISPRTags2xUGI, bpNLS and bpNLSExpressionMammalianAvailable sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBTK1126
Plasmid#236187PurposeBsaI compatible gentamicin resistance cassetteDepositorInsertgentamicin resistance cassette
ExpressionBacterialPromoterCP25Available sinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Reckleen_3plus_sgRNA_ptet_pos5
Plasmid#233460PurposeLevel 1 part, Ptet sfgfp dropout sgRNA for position 5, for building RECKLEEN plasmid with multiple sgRNAsDepositorInsertLevel 1 part, Ptet sfgfp dropout sgRNA for position 5, ColE1 ori, and KanR.
UseCRISPRAvailable sinceApril 24, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3RZY
Plasmid#234156PurposeHeterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coliDepositorAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV isolate PRVABC59 NS3 helicase domain
Plasmid#228664PurposeBacterial expression of the Zika virus NS3 helicaseDepositorInsertZika virus NS3 helicase
TagsHis-GST-TEVExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain
Plasmid#228663PurposeBacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)DepositorInsertZika virus portion of NS2B protease coexpressed with NS3 protease
TagsHis-GST-TEVExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pX330_DDX4-cterm
Plasmid#222520PurposeCas9/sgRNA plasmid for targeting DDX4DepositorPublicationInsertCas9, DDX4 sgRNA (DDX4 Synthetic, Human)
UseCRISPRAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227923PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRPL36 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227907PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227910PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertGOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227904PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHW90004
Plasmid#222436PurposeLevel 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.DepositorInsertCBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
UseSynthetic BiologyExpressionPlantMutationnoAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-SunTag(10X)-2A-BFP
Plasmid#227389PurposeAdhesion module, dCas9-SunTagDepositorInsertdCas9-SunTag
UseLentiviralMutationEnzymatically dead Cas9Available sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-GFP-iLID-Ferritin
Plasmid#227376PurposeCondensate module, light-inducible oligomerization 'core'DepositorInsertGFP 'core'
UseLentiviralAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BLU-VIPR
Plasmid#220497PurposeRetroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR and RetroviralExpressionMammalianAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DVA_p15A_AF
Plasmid#217604PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_p15A_AF
Plasmid#217588PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1047
Plasmid#222726PurposepNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NANOS3_cterm
Plasmid#222904PurposeCas9/sgRNA plasmid for targeting NANOS3DepositorPublicationInsertCas9, NANOS3 sgRNA (NANOS3 Synthetic, Human)
UseCRISPRAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv4
Plasmid#221432PurposeLentiviral CRISPR/Cas9 vector for co-expression of sgRNAs and Cas9 protein.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by