We narrowed to 9,521 results for: try

1. pEN_TT 3xFLAG LacZ

   Plasmid

   #83093

   Purpose

   Gateway entry vector for inducible expression of LacZ with N-terminal 3xFLAG tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   LacZ
   

   Use

   Entry vector for gateway cloning

   Tags

   3xFLAG

   Promoter

   TRE tight promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pENTR-R4R3-mRuby-N

   Plasmid

   #113752

   Purpose

   Gateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mRuby2
   

   Use

   CRISPR; Gateway entry vector

   Tags

   mRuby2

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-R4R3-2xmEGFP-N

   Plasmid

   #113747

   Purpose

   Gateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mEGFP-mEGFP
   

   Use

   CRISPR; Gateway entry vector

   Tags

   2xmEGFP

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-R4R3-3xmEGFP-N

   Plasmid

   #113748

   Purpose

   Gateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mEGFP-mEGFP-mEGFP
   

   Use

   CRISPR; Gateway entry vector

   Tags

   3xmEGFP

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-R4R3-2xmEGFP-C

   Plasmid

   #113750

   Purpose

   Gateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mEGFP-mEGFP
   

   Use

   CRISPR; Gateway entry vector

   Tags

   2xmEGFP

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-R4R3-2xmRuby-N

   Plasmid

   #113753

   Purpose

   Gateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mRuby2-mRuby2
   

   Use

   CRISPR; Gateway entry vector

   Tags

   2xmRuby2

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-R4R3-2xmRuby-C

   Plasmid

   #113756

   Purpose

   Gateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   mRuby2-mRuby2
   

   Use

   CRISPR; Gateway entry vector

   Tags

   2xmRuby2

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. RNAPII_CTD27-52_S1966C

   Plasmid

   #98681

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966C

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S1966C

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUPER-retro-puro-shNup88-XbaI

   Plasmid

   #87328

   Purpose

   To express shRNA against human Nup88

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup88
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a - MPX

    Plasmid

    #89619

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Mitocondrial Tryparedoxin peroxidase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR221-CMV.Pro

    Plasmid

    #79486

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    CMV Promoter
    

    Use

    Multisite gateway entry clone

    Promoter

    CMV Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSUPER-retro-puro-shNup93-XhoI

    Plasmid

    #87330

    Purpose

    To express shRNA against human Nup93

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup93
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFOSGE_DUM1B01

    Plasmid

    #71089

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Clk (Clk Fly)
    

    Use

    Gateway entry vector

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pJK278

    Plasmid

    #71134

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-89 to asparagine; additional N-…

    Promoter

    T7

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK130

    Plasmid

    #71129

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed valine-319 to phenylalanine

    Promoter

    T7

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK173

    Plasmid

    #71831

    Purpose

    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genes

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFOSGE_COF2C10

    Plasmid

    #70927

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    okr (okr Fly)
    

    Use

    Gateway entry vector

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pJK320

    Plasmid

    #71144

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-59 to serine; additional N-term…

    Promoter

    T7

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK319

    Plasmid

    #71143

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-59 to alanine; additional N-ter…

    Promoter

    T7

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK318

    Plasmid

    #71142

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-59 to glutamine; additional N-t…

    Promoter

    T7

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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