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PB-BE3-3NLS

Plasmid

#119032

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2689_pCAG-PV1-iCre-N229-L1-nMag-NLS-BGHpA

Plasmid

#108817

Purpose

Expresses N-terminus to aa229 of Cre recombinase fused to nMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa229

Use

Cre/Lox and Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2690_pCAG-PV1-pMag-L1-iCre-230C-BGHpA

Plasmid

#108818

Purpose

Expresses aa230 to C-terminus of Cre recombinase fused to pMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa230 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and pMag

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2210_pCAG-PV1-iCre-N251-L1-GID1-NLS-BGHpA

Plasmid

#108725

Purpose

Expresses N-terminus to aa251 of Cre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa251

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pbIG-Xph20-ETWV

Plasmid

#133034

Purpose

Expresses Xph20-ETWV in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Tags

AviTag, His10, and TEV protease cleavage site

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpA

Plasmid

#108815

Purpose

Expresses N-terminus to aa468 of Bxb1 integrase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Bxb1 integrase fragment N-terminus to aa468

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2678_pCAG-PV1-pMag-L1-FlpO-397C-NLS-BGHpA

Plasmid

#108832

Purpose

Expresses aa397 to C-terminus of Flp recombinase fused to pMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa397 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and pMag

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2585_pCAG-PV1-VCre-N269-L1-ABI-NLS-BGHpA

Plasmid

#108785

Purpose

Expresses N-terminus to aa269 of VCre recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment N-terminus to aa269

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2589_pCAG-PV1-PYL-L1-VCre-270C-NLS-BGHpA

Plasmid

#108786

Purpose

Expresses aa270 to C-terminus of VCre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment aa270 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 D444-630

Plasmid

#19840

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D444-630 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 444-630

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
KHBD00054

Plasmid

#34216

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2275 (Jra Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
FUGW-EGFP-mPkm2-T328E

Plasmid

#242814

Purpose

Lentiviral vector expressing mouse Pkm2-T328E mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

pyruvate kinase, muscle (Pkm Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T328E

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mPkm2-T328A

Plasmid

#242813

Purpose

Lentiviral vector expressing mouse Pkm2-T328A mutant tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

pyruvate kinase, muscle (Pkm Mouse)

Use

Lentiviral

Tags

EGFP

Mutation

T328A

Promoter

Ubiquitin C

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-AMLn-CAGEn-NES (Npu)

Plasmid

#241407

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Extended Figure 3 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

AMLn-CAGEn-NES (Npu) (RUNX1 Synthetic, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII-TRPV1-flag

Plasmid

#238924

Purpose

Expression of the human TRPV1 channel with a Flag-tag in excitatory neurons

Depositor

Vsevolod Belousov

Article

Maltsev et al Cell Mol Life Sci. 2024 Oct 25;81(1):437

Insert

CaMKII-TRPV1-flag

Use

AAV

Tags

flag

Expression

Mammalian

Promoter

CaMKIIalfa

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV TRE-Tight CheRiff-mKate2

Plasmid

#182493

Purpose

Expression of CheRiff channelrhodopsin linked to mKate2 from a Tet-inducible promoter

Depositor

Thomas Oertner

Article

Lamothe-Molina et al Nat Commun. 2022 Oct 26;13(1):6376. doi:

Insert

CheRiff (#51697 Adam Cohen Lab)

Use

AAV

Tags

mKate2 (Evrogen)

Expression

Mammalian

Promoter

TRE-Tight (Clontech)

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-RUNX1-4A

Plasmid

#238534

Purpose

Used for overexpression of human RUNX1 in mammalian cells. Contains mutations that prevent phosphorylation at some sites of the translated protein.

Depositor

Diane Krause

Article

Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do

Inserts

RUNX1 (RUNX1 Human)
Puromycin

Use

Lentiviral

Expression

Mammalian

Mutation

S249A, S266A, T273A, S276A

Promoter

MSCV and PGK

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-RUNX1-4D

Plasmid

#238535

Purpose

Used for overexpression of human RUNX1 in mammalian cells. Contains RUNX1 phosphomimetic mutations.

Depositor

Diane Krause

Article

Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do

Inserts

RUNX1 (RUNX1 Human)
Puromycin

Use

Lentiviral

Expression

Mammalian

Mutation

S249D, S266D, T273D, S276D

Promoter

MSCV and PGK

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET28:LanIV-Streptomyces

Plasmid

#226250

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Streptomyces)

Tags

His6

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2C

Plasmid

#225070

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2C)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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