We narrowed to 13,713 results for: cas9

1. pCMV-MMLVgag-D3A-3L-dCas9-KOX1

   Plasmid

   #240532

   Purpose

   Expresses MMLVgag–DNMT3A-3L-dCas9-KOX1 for producing RENDER-DNMT3A-3L-dCas9-KOX1

   Depositor

   James Nuñez

   Article

   Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

   Insert

   MMLVgag-D3A-3L-dCas9-KOX1
   

   Use

   Tags

   FLAG, HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-E14-ABE-Cas9n-puro

   Plasmid

   #226588

   Purpose

   Express E14-ABE in mammalian cells

   Depositor

   Gang Wang

   Article

   Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

   Insert

   E14-ABE
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. nzCas9n-GSG-linker-P2A-mNeongreen

   Plasmid

   #241189

   Purpose

   Middle entry vector for mini-Golden subcloning platform; mNeongreen

   Depositor

   Junsu Kang

   Insert

   mNeongreen
   

   Use

   Mini-golden

   Tags

   Expression

   Mutation

   TCGC linker

   Promoter

   Available since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

   Plasmid

   #210391

   Purpose

   AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)

   Depositor

   Benjamin Kleinstiver

   Article

   Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:

   Insert

   AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII promoter for Cre, human U6 promoter for Sp…

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. iC30 PB-Zim3-dCas9-P2A-BFP_hygro

   Plasmid

   #236743

   Purpose

   Constitutive expression of Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
   EF1a-hygro-WPRE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and EF1a

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

   Plasmid

   #223111

   Purpose

   pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-SpCas9-P2A-EGFP (MSP2582)

   Plasmid

   #223067

   Purpose

   pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized SpCas9-P2A-EGFP
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Cas9-P2A-dTomato-PS9

   Plasmid

   #231911

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Cas9-P2A-dTomato-T20

   Plasmid

   #231912

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid

    #228576

    Purpose

    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    BRD4 gRNA (BRD4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223323

    Purpose

    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Insert

    SpCas9 and sgRNA-T2 (cas9 )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9 fused to scFv (anti-spike)

    Plasmid

    #186421

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
    

    Use

    CRISPR

    Tags

    6His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

    Plasmid

    #222107

    Purpose

    Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

    Depositor

    Jonathan Weissman

    Article

    She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

    Insert

    CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
    

    Use

    AAV and CRISPR

    Tags

    HA and P2A-mCherry

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1_dCas9-XTEN-KRAB-p2A-GFP

    Plasmid

    #222108

    Purpose

    Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

    Depositor

    Jonathan Weissman

    Article

    She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

    Insert

    CRISPRi-kox1(KRAB) (cas9 Human, S. pyogenes)
    

    Use

    AAV and CRISPR

    Tags

    HA and P2A-GFP

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBK1302-AAV-EFSNC-dCjCas9-KRAB

    Plasmid

    #223147

    Purpose

    Expression of KRAB with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK1312-AAV-EFSNC-dSaCas9-KRAB

    Plasmid

    #223157

    Purpose

    Expression of KRAB with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

    Plasmid

    #220494

    Purpose

    To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting LIG4 exon 3 (LIG4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

    Plasmid

    #209197

    Purpose

    Mutagenesis of Faah

    Depositor

    Larry Zweifel

    Article

    Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

    Insert

    Faah (Faah Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

    Plasmid

    #209199

    Purpose

    Mutagenesis of Kcnma1

    Depositor

    Larry Zweifel

    Article

    Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

    Insert

    Kcnma1 (Kcnma1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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