We narrowed to 6,807 results for: pcdna3.1

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pcDNA3.1-AntiHer2-LC122-TAG

Plasmid

#226839

Purpose

Contains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 122 of light chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AntiHer2-HC197-TAG

Plasmid

#226838

Purpose

Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 197 of heavy chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(P29S)

Plasmid

#224280

Purpose

Cell transfection and expression of Rac1 (P29S)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

P29S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C)

Plasmid

#224279

Purpose

Cell transfection and expression of Rac1 (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

G12C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tras light chain

Plasmid

#216294

Purpose

Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Tras light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pert Light chain

Plasmid

#216310

Purpose

Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Pert Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8

Plasmid

#221683

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-H2-18 Heavy chain-SpyTag003

Plasmid

#216307

Purpose

Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

H2-18 Heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PhLP1-myc-6xHis

Plasmid

#218176

Purpose

Expresses phosducin-like protein 1 in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

Phosducin like protein 1 (PDCL Human)

Tags

6x His and Myc

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

Plasmid

#209851

Purpose

Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

Depositor

Volker Busskamp

Article

Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

Insert

light-driven sodium pump ErNaR

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TST-EGFP-GPIDAF

Plasmid

#213712

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gα13-125-NLuc

Plasmid

#207155

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNA13 (GNA13 Human)

Expression

Mammalian

Mutation

NLuc inserted at position F(125)D

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gα12-133-NLuc

Plasmid

#207156

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNA12 (GNA12 Human)

Expression

Mammalian

Mutation

NLuc inserted at position A(133)F

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a WT pcDNA3.1 Zeo (+)

Plasmid

#206079

Purpose

Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-EGFP

Plasmid

#198512

Purpose

Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Tags

EGFP and Rho1D4

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRoquin1_510-1133_AE

Plasmid

#148613

Purpose

Mammalian Expression of HsRoquin1_510-1133

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1_510-1133 (RC3H1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsMex3B_230-569_AD

Plasmid

#148480

Purpose

Mammalian Expression of HsMex3B_230-569

Depositor

Elisa Izaurralde

Insert

HsMex3B_230-569 (MEX3B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-beta-hRLuc-V5-6xbsMS2_V

Plasmid

#147738

Purpose

Mammalian Expression of Rluc-6xbsMS2

Depositor

Elisa Izaurralde

Insert

Rluc-6xbsMS2

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tau Authentic Alu 10-12V337M

Plasmid

#194169

Purpose

Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-associated protein tau 10-12 V337M

Tags

3X flag

Expression

Mammalian

Mutation

Changed Valine 337 to Methinonine

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro-Mfn2-ActA

Plasmid

#191981

Purpose

Expression of Mfn2 tagged with mitochondrion localization signal (ActA)

Depositor

Luca Scorrano

Insert

Mfn2-ActA

Tags

ActA

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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