We narrowed to 22,140 results for: his
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Plasmid#146075PurposeInsect Expression of DmTral-LSm-R42EF44ADepositorInsertDmTral-LSm-R42EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-S13AI15A-V5His6_C
Plasmid#146076PurposeInsect Expression of DmTral-LSm-S13AI15ADepositorInsertDmTral-LSm-S13AI15A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-S70AD71K-V5His6_C
Plasmid#146077PurposeInsect Expression of DmTral-LSm-S70AD71KDepositorInsertDmTral-LSm-S70AD71K (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-F44A-V5His6_C
Plasmid#146047PurposeInsect Expression of DmTral-LSm-F44ADepositorInsertDmTral-LSm-F44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EE23KR42EF44A-V5His6_C
Plasmid#146048PurposeInsect Expression of DmTral-LSm-R21EE23KR42EF44ADepositorInsertDmTral-LSm-R21EE23KR42EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EE23K-V5His6_C
Plasmid#146050PurposeInsect Expression of DmTral-LSm-R21EE23KDepositorInsertDmTral-LSm-R21EE23K (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EF44A-V5His6_C
Plasmid#146051PurposeInsect Expression of DmTral-LSm-R21EF44ADepositorInsertDmTral-LSm-R21EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21ER42EF44A-V5His6_C
Plasmid#146052PurposeInsect Expression of DmTral-LSm-R21ER42EF44ADepositorInsertDmTral-LSm-R21ER42EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21ES13AI15A-V5His6_C
Plasmid#146053PurposeInsect Expression of DmTral-LSm-R21ES13AI15ADepositorInsertDmTral-LSm-R21ES13AI15A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R42EF44A-V5His6_C
Plasmid#146054PurposeInsect Expression of DmTral-LSm-R42EF44ADepositorInsertDmTral-LSm-R42EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-S13AI15A-V5His6_C
Plasmid#146055PurposeInsect Expression of DmTral-LSm-S13AI15ADepositorInsertDmTral-LSm-S13AI15A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-D30KT35A-V5His6_C
Plasmid#146067PurposeInsect Expression of DmTral-LSm-D30KT35ADepositorInsertDmTral-LSm-D30KT35A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-del46-60-V5His6_C
Plasmid#146068PurposeInsect Expression of DmTral-LSm-del46-60DepositorAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-F44A-V5His6_C
Plasmid#146069PurposeInsect Expression of DmTral-LSm-F44ADepositorInsertDmTral-LSm-F44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-K73AD74A-V5His6_C
Plasmid#146070PurposeInsect Expression of DmTral-LSm-K73AD74ADepositorInsertDmTral-LSm-K73AD74A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KS13AI15A-V5His6_C
Plasmid#146072PurposeInsect Expression of DmTral-LSm-R21EE23KS13AI15ADepositorInsertDmTral-LSm-R21EE23KS13AI15A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C
Plasmid#146046PurposeInsect Expression of DmTral-LSm-del46-60DepositorAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C
Plasmid#146006PurposeInsect Expression of DmSmaug-del584-762DepositorInsertDmSmaug-del584-762 (smg Fly)
UseTagsExpressionInsectMutation7 silent mutations and a deletion of the AA 961-9…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSmaug-del_584-762-V5His6_C
Plasmid#146015PurposeInsect Expression of DmSmaug-del584-762DepositorInsertDmSmaug-del584-762 (smg Fly)
UseTagsExpressionInsectMutation7 silent mutations and a deletion of the AA 961-9…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF3-K31E-siRNAres-V5His6_N
Plasmid#147007PurposeInsect Expression of DmUPF3-K31E-siRNAresDepositorInsertDmUPF3-K31E-siRNAres (Upf3 Fly)
UseTagsExpressionInsectMutation5 silent and one non silent mutation P305S compar…PromoterAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N
Plasmid#146990PurposeInsect Expression of DmUPF1_92-1180-siRNAresDepositorInsertDmUPF1_92-1180-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_1-417-Y342AL347A-V5His6_M
Plasmid#146901PurposeInsect Expression of DmCup_1-417-Y342AL347ADepositorAvailable SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-tubulin(N229A)-TEV-His6
Plasmid#178077PurposeInsect cell expression of gamma-tubulin(N229A)-TEV-His6DepositorInsertgamma-tubulin with an N229A point mutation (TUBG1 Human)
UseTagsTEV-His6ExpressionInsectMutationChanged Asparagine 229 to AlaninePromoterAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K569A,K571A)-StrepII
Plasmid#174426PurposeExpresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK569A, K571APromoterT7Available SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Fusion + CBP TAZ1(340-439)
Plasmid#173762PurposeBacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-FusionL21A + CBP TAZ1(340-439)
Plasmid#173763PurposeBacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIsGB1-FusionL63A + CBP TAZ1(340-439)
Plasmid#173764PurposeBacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructsDepositorUseTagsHis6GB1 and thrombinExpressionBacterialMutationfusion of CITED2 and Hif1aPromoterT7Available SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis
Plasmid#176573PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184QPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q W330Y)-6xHis
Plasmid#176577PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184Q W330YPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K557A,K560A)-StrepII
Plasmid#174425PurposeExpresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK557A, K560APromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin-FLN-ELP-His-ybbr
Plasmid#168039PurposeExpresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …ExpressionBacterialMutationPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-FLN-ELP-His-ybbr
Plasmid#168047PurposeExpresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, ddFLN4, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C
Plasmid#146071PurposeInsect Expression of DmTral-LSmR21EE23KR42EF44ADepositorInsertDmTral-LSmR21EE23KR42EF44A (tral Fly)
UseTagsExpressionInsectMutationR21E, E23K, R42E, F44A. Additionally, one silent …PromoterAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C
Plasmid#146049PurposeInsect Expression of DmTral-LSmR21EE23KS13AI15ADepositorInsertDmTral-LSmR21EE23KS13AI15A (tral Fly)
UseTagsExpressionInsectMutationS13A, I15A, R21E, E23K, one silent mutation A198C…PromoterAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700]-Ss(424)
Plasmid#166852PurposeExpresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.DepositorAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-His-SpyTag-XMod(XL2V)-Doc
Plasmid#157676PurposeExpression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.DepositorInsertXMod-Doc mutant XL2V with Histag and SpyTag
UseTagsHistag and SpyTagExpressionBacterialMutationPromoterT5Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7pr-His6-MBP-TEV-Cas9-BirA*
Plasmid#159993PurposeBacterial expression of Cas9-BirA*DepositorInsertCas9-BirA*
UseTags6X-His, MBP, 3X-FLAG, NLSExpressionBacterialMutationD10A, H840APromoterT7Available SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS
Plasmid#153443PurposeE. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
UseTags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-His-MS2BP-G3BP1-S149A
Plasmid#136009PurposeS149A G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)DepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
IX401: pMVP (L3-L2) 6x His epitope tag + polyA
Plasmid#121754PurposepMVP L3-L2 entry plasmid, contains 6x His epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsert6x His epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only