We narrowed to 3,022 results for: ER

1. pLenti-CaMKIIa-eNpHR 3.0-EYFP

   Plasmid

   #26970

   Purpose

   3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Insert

   eNpHR 3.0
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Trafficking Signal (TS), ER Export Signal (FCYENE)

   Promoter

   CaMKIIa

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-35aaNSP1SA11-Green Lantern(wt)

   Plasmid

   #220821

   Purpose

   Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the Green Lantern wt protein carrying an ER-localization signal at the N- and C-termini

   Depositor

   Alex Borodavka

   Insert

   Rotavirus SA11 35aaNSP1-Green Latern(wt)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-35aaNSP1SA11-Green Lantern(io)

   Plasmid

   #220822

   Purpose

   Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the recoded Green Lantern protein carrying an ER-localization signal at the N- and C-termini

   Depositor

   Alex Borodavka

   Insert

   Rotavirus SA11 35aaNSP1-Green Latern(io)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

   Plasmid

   #162377

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   Synapsin

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

   Plasmid

   #185912

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpRY-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

   Plasmid

   #197510

   Purpose

   Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and NpuN(intein)-BPNLS

   Expression

   Mutation

   ABE8e mutations in TadA and nSpRY(D10A/A61R)

   Promoter

   Cbh

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

   Plasmid

   #197511

   Purpose

   Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and BPNLS-NpuC(intein)

   Expression

   Mutation

   C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

   Promoter

   Cbh

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

   Plasmid

   #197503

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

   Plasmid

   #197501

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP-AAV-syn-jGCaMP7f-WPRE

    Plasmid

    #104488

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

    Insert

    jGCaMP7f
    

    Use

    AAV

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-kz-CD20-Puro

    Plasmid

    #209759

    Purpose

    Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

    Plasmid

    #208289

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA; mutations in iSpyMac

    Promoter

    CMV and T7

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid

    #185917

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

    Plasmid

    #162380

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CAG

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid

    #197499

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162378

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV(MinDis).iGluSnFR

    Plasmid

    #41732

    Purpose

    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Insert

    GltI based Glutamate Biosensor
    

    Use

    Glutamate biosensor

    Tags

    Myc, PDGFR transmembrane helix (aa 513-561), and …

    Expression

    Mammalian

    Mutation

    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

    Promoter

    CMV

    Available since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-AAV-syn-jGCaMP7s-WPRE

    Plasmid

    #104487

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP7s
    

    Use

    AAV

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET-RCaMP1h

    Plasmid

    #42874

    Purpose

    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

    Depositor

    Loren Looger

    Article

    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

    Insert

    Red Fluorescent Calcium binding Protein
    

    Use

    Tags

    EK (Enterokinase) Recognition Site, His Tag (6x),…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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