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pPSdown2

Plasmid

#149419

Purpose

Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted downstream of the GFP reporter gene.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Type

Empty backbone

Expression

Plant

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-proinsulin-mEGFP

Plasmid

#160464

Purpose

Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

proinsulin-mEGFP

Expression

Bacterial

Promoter

T5

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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bRA90

Plasmid

#100951

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mito

Plasmid

#71542

Purpose

Biomedical Research

Depositor

David Sidransky

Article

Dasgupta et al Cancer Res. 2008 Feb 1;68(3):700-6. doi:

Insert

myc-mito

Use

Cytomegalovirus

Tags

none

Promoter

CMV

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
bRA77

Plasmid

#100953

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMB70

Plasmid

#47943

Purpose

Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

guide RNA

Use

CRISPR

Expression

Worm

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pScDD2_ERdd

Plasmid

#109047

Purpose

destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

Depositor

Thomas Wandless

Insert

ER50 (ESR1 H. sapiens gene with yeast enhanced codon)

Tags

yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

Expression

Yeast

Mutation

3 mutations : E380G, R434G, N532S

Promoter

SOD1

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMOTag-glmS-4H

Plasmid

#106378

Purpose

Can be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS-3xHA

Available Since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_HAIB

Plasmid

#184869

Purpose

For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a_LolT_C41S

Plasmid

#211789

Purpose

Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S

Depositor

Yang Hai

Article

Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:

Insert

lolt mutant

Tags

His tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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