We narrowed to 5,443 results for: SPI

1. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

   Plasmid

   #224505

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   LexA-mODC-LexOP-35SCaMV
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

   Plasmid

   #224506

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   LexA-oMDC-LexOP-c-Fos
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

   Plasmid

   #224512

   Purpose

   A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   3xHA-BirA-T2A-mCherry-Caax
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

   Plasmid

   #224522

   Purpose

   A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458-SPNS1-sg1

   Plasmid

   #198566

   Purpose

   SPNS1-targeting sgRNA in PX458 vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1-sg1 (SPNS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458-SPNS1-sg2

   Plasmid

   #198567

   Purpose

   SPNS1-targeting sgRNA in PX458 vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1-sg2 (SPNS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1-Cdc45-6His

   Plasmid

   #211379

   Purpose

   bacterial expression of human Cdc45 C-terminally fused to 6xHis-Tag

   Depositor

   Helmut Pospiech

   Article

   Szambowska et al Nucleic Acids Res. 2014 Feb;42(4):2308-1

   Insert

   Cdc45 (CDC45 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_NFL(K468TAG)-FLAG

   Plasmid

   #182658

   Purpose

   Expresses mouse neurofilament light chain with a TAG codon at the position K468 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   mouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
   

   Use

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   K468TAG

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_NFL(K211TAG)-FLAG

   Plasmid

   #182655

   Purpose

   Expresses mouse neurofilament light chain with a TAG codon at the position K211 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
   

   Use

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   K211TAG

   Promoter

   CMV

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_NFL(R438TAG)-FLAG

    Plasmid

    #182657

    Purpose

    Expresses mouse neurofilament light chain with a TAG codon at the position R438 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
    

    Use

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    R438TAG

    Promoter

    CMV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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