Skip to main content
Addgene
Showing: 6201 - 6250 of 6372 results

  1. pMN-015

    Plasmid
    #195419
    Purpose
    AAVS1 Integratable mCherry Reporter for synZiFTR ZF7
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    4X ZF7 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195419-plasmid-map-sequence-id-384990

    Enlarge

  2. pMN-016

    Plasmid
    #195420
    Purpose
    AAVS1 Integratable mCherry Reporter for synZiFTR ZF8
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    4X ZF8 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195420-plasmid-map-sequence-id-384999

    Enlarge

  3. pMN-017

    Plasmid
    #195421
    Purpose
    AAVS1 Integratable mCherry Reporter for synZiFTR ZF9
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    4X ZF9 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195421-plasmid-map-sequence-id-384992

    Enlarge

  4. pMN-019

    Plasmid
    #195423
    Purpose
    AAVS1 Integratable mCherry Reporter for synZiFTR ZF11
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    4X ZF11 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195423-plasmid-map-sequence-id-384882

    Enlarge

  15. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Use
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV and gene trap
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

    Enlarge

  16. pCLV3Cas-LT1

    Plasmid
    #210549
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
    Use
    Tags
    Expression
    Plant
    Mutation
    2bp insertion after AAVS1 target sequence that di…
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    210549-plasmid-map-sequence-id-426236

    Enlarge

  17. MTK0_051

    Plasmid
    #123948
    Purpose
    Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hAAVS1 landing pad destination vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123948-plasmid-map-sequence-id-250127

    Enlarge

  18. MTK0_057

    Plasmid
    #123950
    Purpose
    Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    BxBI LP on hAAVS1 (Hygro::mRuby)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    123950-plasmid-map-sequence-id-271459

    Enlarge

  19. pCLV3Cas-LT2

    Plasmid
    #210550
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
    Use
    Tags
    Expression
    Plant
    Mutation
    1bp insertion after AAVS1 target sequence that di…
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    210550-plasmid-map-sequence-id-426233

    Enlarge

  39. pEMS1424

    Plasmid
    #29220
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    Nov. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
    29220-plasmid-map-sequence-id-13635

    Enlarge

  40. pEMS1062

    Plasmid
    #29011
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-Cre/EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    Feb. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    29011-plasmid-map-sequence-id-13417

    Enlarge

  41. pEMS1626

    Plasmid
    #29281
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-Intron-LacZ-NLS
    Use
    High copy number homologous recombination plasmid…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    June 10, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29281-plasmid-map-sequence-id-13696

    Enlarge

  42. LPUtopia-7

    Plasmid
    #199212
    Purpose
    Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Inserts
    aminoglycoside phosphotransferase,
    enhanced Green Fluorescence Protein
    Red Fluorescence Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and thymidine kinase promoter
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    199212-plasmid-map-sequence-id-396462

    Enlarge

  43. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103871-plasmid-map-sequence-id-259670

    Enlarge

  44. KRD22_HBA1(HBA1reg-HBB-longHAs)

    Plasmid
    #232403
    Purpose
    AAV production plasmid for HBA1 long HAs vector from Figs. 3-6 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank full HBA1-2A-YFP cassette. HAs are ~900bp
    Depositor
    Kyle Cromer, Matthew Porteus
    Article
    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10
    Inserts
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Human Hemoglobin Subunit Beta (HBB Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232403-plasmid-map-sequence-id-466149

    Enlarge

  45. hSyn1-FLEX-hChR2-tdtomato

    Plasmid
    #41015
    Depositor
    Karel Svoboda
    Insert
    Channelrhodopsin-2
    Use
    Adenoviral
    Tags
    tdtomato
    Expression
    Mammalian
    Mutation
    codon-optimized (human)
    Promoter
    synapsin
    Available since
    Nov. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  46. mTLR4 flag

    Plasmid
    #13087
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (Tlr4 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-19 have been replaced with mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13087-plasmid-map-sequence-id-147760

    Enlarge

  47. hTLR2 flag

    Plasmid
    #13082
    Depositor
    Ruslan Medzhitov
    Insert
    TLR2 (TLR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-16 have been replaced with mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13082-plasmid-map-sequence-id-142444

    Enlarge

  48. hTLR5 flag

    Plasmid
    #13088
    Depositor
    Ruslan Medzhitov
    Insert
    TLR5 (TLR5 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-19 have been replaced by a mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13088-plasmid-map-sequence-id-147761

    Enlarge

  49. hTLR3 flag

    Plasmid
    #13084
    Depositor
    Ruslan Medzhitov
    Insert
    TLR3 (TLR3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-17 have been replaced by mammalian …
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13084-plasmid-map-sequence-id-229543

    Enlarge

  50. hTLR1 flag

    Plasmid
    #13081
    Depositor
    Ruslan Medzhitov
    Insert
    TLR1 (TLR1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-18 have been replaced with mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 6201 - 6250 of 6372 results