We narrowed to 4,446 results for: GCA

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TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-PLXNB2

Plasmid

#69240

Purpose

U6 driven SpCas9 sgRNA expression for PLXNB2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

PLXNB2 sgRNA (PLXNB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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AP334-5

Plasmid

#66096

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
AP334-6

Plasmid

#66097

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP334-2

Plasmid

#66095

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-4

Plasmid

#66094

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-3

Plasmid

#66093

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-2

Plasmid

#66092

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
AP332-4

Plasmid

#66091

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs4

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
AP332-3

Plasmid

#66090

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs4

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
AP332-2

Plasmid

#66089

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs4

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
MS2-adRNA (5'GAC)

Plasmid

#170127

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

Plasmid

#177335

Purpose

bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

Depositor

Christopher Overall

Article

Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

Insert

SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)

Tags

Factor Xa site-3xFlag-Myc-6xHis

Expression

Bacterial

Mutation

silent mutation C to T at position 10546 (elimina…

Promoter

T7

Available Since

Nov. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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DYNLL2 A10.5 gRNA

Plasmid

#90663

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLL2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLL2 (Guide Designation A10.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Actb KI #2

Plasmid

#139666

Purpose

Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_Dual_pegRNA

Plasmid

#173200

Purpose

Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N pegRNA

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-ctrl-guides

Plasmid

#168240

Purpose

"neutrophil specific GFP with ubiquitous ctrl sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

control sgRNAs

Use

CRISPR

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-TUBA1B

Plasmid

#207763

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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