We narrowed to 159,461 results for: addgene

1. WDR4_pCSdest

   Plasmid

   #53953

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   WDR4 (WDR4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pGH183_MS2-AIDΔDead-Hygro

   Plasmid

   #85414

   Purpose

   lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   MS2-AIDΔDead and Hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-ytdTomato-URA3

   Plasmid

   #168054

   Purpose

   Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ytdTomato
   

   Use

   Tags

   ytdTomato

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TMPRSS15_pCSdest

   Plasmid

   #53806

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   TMPRSS15 (TMPRSS15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pX330-NEAT1pr_v1

   Plasmid

   #97081

   Purpose

   Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1pr_v1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15b-17X-PTDH

   Plasmid

   #166786

   Purpose

   Phosphite dehydrogenase for NAD(P)H regeneration

   Depositor

   Wilfred van der Donk

   Article

   Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

   Insert

   Phosphite dehydroenase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   A196R, T201S, A328T, E352N, and C356D- please see…

   Promoter

   T7

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBigTB-CreERT2

   Plasmid

   #149434

   Purpose

   tamoxifen-inducible Cre recombinase

   Depositor

   Primo Schaer

   Article

   Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. M4786 KanMX::hisG-URA3-HisG Disruptor Converter

   Plasmid

   #51690

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   hisG-URA3-hisG (URA3 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TTC3_pCSdest

   Plasmid

   #53851

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   TTC3 (TTC3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pRG714_lexO-AscI_LexA-TF_URA3MX

    Plasmid

    #154822

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. RIB VP64 R1

    Plasmid

    #161493

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-Synapsin-mCherry-C1-WPRE

    Plasmid

    #159956

    Purpose

    untargeted marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mCherry-C1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FU-MAP2-Gateway

    Plasmid

    #43915

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5 and non-standard His tag (HHRHHH)

    Expression

    Mammalian

    Mutation

    Promoter

    Human MAP2 promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid

    #185918

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH-roGFP2-ORP1

    Plasmid

    #191677

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    redox(ro)GFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-NEAT1pr_v2

    Plasmid

    #97082

    Purpose

    Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1pr_v2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. IRES-H2B-YFP-DD

    Plasmid

    #96893

    Purpose

    Reporter to monitor translation of EMCV IRES mRNA

    Depositor

    Tobias Meyer

    Article

    Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

    Insert

    IRES-H2B-Venus-DD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICH-chl-roGFP2-synORP1

    Plasmid

    #191701

    Purpose

    Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-roGFP2-synORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACAGW-oChiEFtdTomato-AAV

    Plasmid

    #32846

    Purpose

    AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

    Depositor

    Rory McQuiston

    Article

    Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

    Insert

    oChiEF
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    CAGG

    Available since

    March 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH-nuc-roGFP2-synORP1

    Plasmid

    #191759

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-synORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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