We narrowed to 142,835 results for: ins

1. pcDNA3-delta133HtrA-EGFP

   Plasmid

   #14124

   Depositor

   L. Miguel Martins

   Article

   Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of the first 133 amino-acids

   Promoter

   Available since

   March 7, 2007

   Availability

   Academic Institutions and Nonprofits only

2. STT3A pEGFP-N2 (1051)

   Plasmid

   #62025

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   STT3A (STT3A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

3. p6600 MSCV-IP N-HAonly JUN

   Plasmid

   #34898

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   JUN (JUN Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pBabepuro3-p16Flag

   Plasmid

   #24934

   Depositor

   Lynda Chin

   Article

   Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

   Insert

   p16 (CDKN2A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSP64-hKCNQ1

   Plasmid

   #53048

   Purpose

   Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

   Insert

   Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHS1950

   Plasmid

   #205983

   Purpose

   Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Inserts

   Ga0116245_10002768 candidate type VII proteins
   Cas14
   Cas5
   CRISPR array
   

   Use

   Tags

   14xHis and TwinStrep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (proteins), pJ23119 (crRNA)

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

   Plasmid

   #157974

   Purpose

   Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

   Depositor

   Carolyn McBride

   Article

   Zhao et al Nature. 2022 May;605(7911):706-712. doi:

   Insert

   Orco
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CRY2olig-mCh-Nck

   Plasmid

   #60034

   Purpose

   Expresses a fusion containing CRY2olig (PHR, E490G) with mCherry and 3 SH3 domains of Nck

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2-mCh-NCK (CRY2 Mustard Weed, Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   M354I, E490G CRY2

   Promoter

   CMV

   Available since

   Nov. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBabepuro3-p18Flag

   Plasmid

   #24933

   Depositor

   Lynda Chin

   Article

   Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

   Insert

   P18 (CDKN2C Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pDONR P2R-P3-EGFP

    Plasmid

    #48349

    Purpose

    Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Aminoacid 40 is valine in our sequence, while it …

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LADL Anchor

    Plasmid

    #127664

    Purpose

    Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    dCas9-CIBN-2A-Puro
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 (D10A,H840A) mutant

    Promoter

    EF1a

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

    Plasmid

    #53551

    Purpose

    mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"

    Depositor

    Kathleen Collins

    Article

    Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    contains aa 88-544, with aa 166-172 replaced wit…

    Promoter

    CMV

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET30a-SARS-CoV2-NP-Nt(47-173)

    Plasmid

    #165091

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pTriex-mVenus-PI-RhoA(Q63L)

    Plasmid

    #86978

    Purpose

    PI(DM): Photo-activatable/light sensitive

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    RhoA (RHOA Human)
    

    Use

    Tags

    6xHis and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q63L

    Promoter

    CMV

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV-MAVS

    Plasmid

    #221313

    Purpose

    Expression of MAVS in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    MAVS (Mavs Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p6608 MSCV-IP N-HAonly CHAF1B

    Plasmid

    #34901

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    CHAF1B (CHAF1B Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-KLF3

    Plasmid

    #49100

    Purpose

    Expresses KLF3 in mammalian cells

    Depositor

    Merlin Crossley

    Article

    Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

    Insert

    Krüppel-Like Factor 3(KLF3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pJW2088

    Plasmid

    #154336

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR P2R-P3-V5-6xHis

    Plasmid

    #48347

    Purpose

    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    V5 and 6xHis epitope tags cassette
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Contains a stop codon at the end of the epitope t…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Igfbp1-promoter/pGL3

    Plasmid

    #12146

    Depositor

    Domenico Accili

    Article

    Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

    Insert

    IGFBP1 promoter (Igfbp1 Rat)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains the canonical insulin response sequence …

    Promoter

    Available since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only