We narrowed to 5,382 results for: kras

1. pENTR221-YAP1

   Plasmid

   #79503

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pProEx Rap1A(G12C, L96F) CL

   Plasmid

   #224264

   Purpose

   Protein purification of Rap1A (G12C, L96F)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rap1A (RAP1A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G12C, L96F

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5FRT/TO-p47-Strep-HA

   Plasmid

   #113475

   Purpose

   Expression of human p47 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   p47 (NSFL1C Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DNMT3L-dCas9

   Plasmid

   #126587

   Purpose

   DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   DNMT3L (DNMT3L Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-DNMT3L-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PET-eSpCas9-plus-NLS-6xHis

   Plasmid

   #207380

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, R1060A, amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTol2CG2-hsp70I:QFGal4-SV40pA

   Plasmid

   #155120

   Purpose

   Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   hsp70l:QFGal4 (hsp70l Budding Yeast, Neurospora crassa, Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   hsp70l

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNH605-PACE-YFP

   Plasmid

   #172002

   Purpose

   Fluorescent reporter for Rpn4 activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xPACE-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1 core promotor with 4 tandem repeats of Rpn4 …

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTol2CG2-acta1:QFGal4-SV40pA

   Plasmid

   #155121

   Purpose

   Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   acta1:QFGal4
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   acta1

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SpCas9 TadCBEa-V106W

   Plasmid

   #193838

   Purpose

   Express TadCBEa-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDa V106W-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DRH335: FCK-Optopatch1

    Plasmid

    #51695

    Purpose

    Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    Optopatch1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p2T-cmv-SpCas9-TadCBEa-BlastR

    Plasmid

    #193847

    Purpose

    Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDF-mTET1

    Plasmid

    #81052

    Purpose

    bacterial expression of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

    Plasmid

    #172514

    Purpose

    Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

    Plasmid

    #159689

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

    Depositor

    Dominic Esposito

    Article

    Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PET-B-eSpCas9-NLS-6xHis

    Plasmid

    #207383

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, amino acids 1005-1013 repl…

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5FRT/TO-VCPIP1-Strep-HA

    Plasmid

    #113482

    Purpose

    Expression of human VCPIP1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    VCPIP1 (VCPIP1 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-CMV-cGAS C396/7A-HA

    Plasmid

    #130917

    Purpose

    Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS C396/7A
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutated residues 396 and 397 to alanines

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJC5-4-insulator-BREreporter-insulator

    Plasmid

    #171499

    Purpose

    injections to make Bmp reporter mouse transgenic line

    Depositor

    James Briscoe

    Article

    Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

    Insert

    Insulator BRE-MLP:H2B-Turqoise
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    BRE-MLP

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

19. pAT15547_PEmax-SuperFi-Cas9

    Plasmid

    #184377

    Purpose

    Mammalian expression of SuperFi-Cas9 PEmax prime editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    PEmax-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS and c-myc NLS

    Expression

    Mammalian

    Mutation

    H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

    Plasmid

    #124860

    Purpose

    Mutagenesis of Slc17a6

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a6 (Slc17a6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge