We narrowed to 4,232 results for: pCMV

1. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

   Plasmid

   #208962

   Purpose

   Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

   Plasmid

   #208945

   Purpose

   A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

   Plasmid

   #210504

   Purpose

   expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
   

   Expression

   Mammalian

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

   Plasmid

   #171616

   Purpose

   Soma-targeted Cal-Light with Kv2.1

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA
   

   Use

   Synthetic Biology

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV5 Flag-Par6 delta 250-295 Flag tagged

   Plasmid

   #24646

   Depositor

   Jeff Wrana

   Article

   Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

   Insert

   Par6c (Pard6a Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of the PDZ domain of Par6c (aa 250-295)

   Available Since

   June 2, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pCMV CHMP2B-L4D,F5D-V82E M85E M92E-3XHA

   Plasmid

   #242418

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with V82E, M85E, and M92E mutations and a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D V82E M85E M92E

   Promoter

   CMV

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV CHMP2B-L4D F5D-d106-113-3xHA (Δα3)

   Plasmid

   #232007

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with the third alpha helix deleted with a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D Δ106-113

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV CHMP2B-L4D F5D-d118-138-3xHA (Δα4)

   Plasmid

   #232008

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fourth alpha helix deleted with a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D Δ118-138

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV CHMP2B-L4D F5D-d55-138-3xHA (Δα2-4)

   Plasmid

   #232009

   Purpose

   Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second through fourth helices deleted with a 3xHA tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   L4D F5D Δ55-138

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV CHMP2B-L4D F5D-d159-174-3xHA (Δα5)

    Plasmid

    #232010

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fifth alpha helix deleted with a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    L4D F5D Δ159-174

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV CHMP2B-L4D F5D-d201-213-HA (ΔVPS4 binding)

    Plasmid

    #232011

    Purpose

    Expression of a CHMP2B membrane binding mutant (L4D F5D) with the VPS4-binding region deleted with a 3xHA tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L4D F5D Δ201-213

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

    Plasmid

    #225064

    Purpose

    Expression of HalM-modified HalA2 in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    HalA2
    

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-AN-DDK_WT RAD23A_aa 111-363 (delta UBL domain)

    Plasmid

    #201460

    Purpose

    Expresses a mutant form of human RAD23A that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks N-terminal residues 1-110

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-AN-DDK_WT RAD23A_delta aa 161-201 (delta UBA1 domain)

    Plasmid

    #201463

    Purpose

    Expresses a mutant form of human RAD23A that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 161-201

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

    Plasmid

    #201546

    Purpose

    Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 188-228

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

    Plasmid

    #192835

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Tags

    3xFLAG-PreScission cleavage site and 6xhis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

    Plasmid

    #185701

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(FCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

    Plasmid

    #185705

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PDCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

    Plasmid

    #185703

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(SCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

    Plasmid

    #185704

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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