Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,324 results for: bli

Showing: 5001 - 5040 of 9324 results

  1. pLV-Enh eGFP Reporter-muEDC-Int3

    Plasmid
    #59288
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the mouse Epidermal Differentiation Complex.
    Depositor
    Benjamin Yu
    Insert
    mFlg int-Intron 1
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. p14-9VNA5

    Plasmid
    #50931
    Purpose
    Expresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Amino acids 96-100 of SRP14 changed to alanine
    Promoter
    CMV
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pPyCAG-Nanog(Y136A)-IP

    Plasmid
    #62995
    Purpose
    Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y136A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 136 (137 AA in Mouse Protein) to…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62995-plasmid-map-sequence-id-104597

    Enlarge

  4. CSNK1G2

    Plasmid
    #39150
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CSNK1G2 (CSNK1G2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)

    Plasmid
    #44096
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available Since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44096-plasmid-map-sequence-id-61688

    Enlarge

  6. pIRES-Neo-HA-cdc25C1

    Plasmid
    #40966
    Depositor
    Olivier Gavet
    Insert
    cdc25C5 (CDC25C Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50626
    Purpose
    pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50626-plasmid-map-sequence-id-79595

    Enlarge

  9. SLC9A3R2

    Plasmid
    #38948
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLC9A3R2 (NHERF2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

    Enlarge

  13. pUASP Flag Rab32 WT

    Plasmid
    #44833
    Depositor
    Matthew Scott
    Insert
    Rab32 (Rab32 Fly)
    Use
    Drosophila
    Tags
    Flag
    Expression
    Insect
    Available Since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. ENOSF1

    Plasmid
    #39104
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ENOSF1 (ENOSF1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pIRES-Neo-HA-cdc25C5

    Plasmid
    #40967
    Depositor
    Olivier Gavet
    Insert
    cdc25C5 (CDC25C Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. BC-GPRC

    Plasmid
    #44199
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    C-terminal fragment of GPR56 starting at amino a…
    Promoter
    LTR
    Available Since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44199-plasmid-map-sequence-id-60058

    Enlarge

  17. pTF260 (pFA6a-PSc-6xGly-KanMX)

    Plasmid
    #44086
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44086-plasmid-map-sequence-id-61663

    Enlarge

  18. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73317-plasmid-map-sequence-id-140432

    Enlarge

  19. pUC57-sgDedd-1

    Plasmid
    #74435
    Purpose
    sgRNA expression vector for mouse Dedd gene targeting intron 5
    Depositor
    Petra Arck
    Insert
    Dedd (Dedd Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pPyCAG-Nanog(K137A)-IP

    Plasmid
    #62996
    Purpose
    Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K137A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Lysine 137 (138 AA in Mouse Protein) to A…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62996-plasmid-map-sequence-id-104598

    Enlarge

  21. pPyCAG-Nanog(Y119A)-IP

    Plasmid
    #62991
    Purpose
    Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y119A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 119 (120 AA in Mouse Protein) to…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62991-plasmid-map-sequence-id-104440

    Enlarge

  22. pIRES-GFP-hFJX1-myc

    Plasmid
    #46928
    Purpose
    coexpression of FJX1-myc and GFP in mammalian cells
    Depositor
    R.D. Beauchamp, Natasha Deane
    Insert
    FJX1 (FJX1 Human)
    Tags
    myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 13, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. ACBD4

    Plasmid
    #39009
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACBD4 (ACBD4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. pUASp YFP Rholike CA

    Plasmid
    #52241
    Purpose
    Drosophila expression of YFP-Rholike constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rholike (RhoL Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q69L; CA mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. 4LG9

    Plasmid
    #60096
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TBL1XR1:APC048-A06:C27585 (TBL1XR1 Human, TBL1XR1:APC048-A06:C27585)
    Use
    Baculovirus expression
    Tags
    6xHis
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. pHygroMod-Foxd3-3xFlag

    Plasmid
    #37274
    Depositor
    Patricia Labosky
    Insert
    Foxd3 (Foxd3 Mouse)
    Use
    Mouse Targeting; Insertion vector for rmce
    Tags
    Flag tag on Foxd3
    Available Since
    July 31, 2012
    Availability
    Academic Institutions and Nonprofits only

  29. TDRD5

    Plasmid
    #32869
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD5 (TDRD5 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. pLEX_305_CIC(ex18)-NUTM1(ex3)

    Plasmid
    #247363
    Purpose
    Lentiviral constitutive expression construct for CIC(ex18)-NUTM1(ex3) (pLEX_305 backbone)
    Depositor
    Ross Okimoto
    Insert
    CIC(ex18)::NUTM1(ex3)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Fusion between CIC (ex18) and NUTM1 (ex3)
    Promoter
    hPGK
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247363-plasmid-map-sequence-id-491583

    Enlarge

  31. pLEX_305_CIC(ex20)-NUTM1(ex6)

    Plasmid
    #247362
    Purpose
    Lentiviral constitutive expression construct for CIC(ex20)::NUTM1(ex6) (pLEX_305 backbone)
    Depositor
    Ross Okimoto
    Insert
    CIC(ex20)::NUTM1(ex6)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Fusion between CIC (ex20) and NUTM1 (ex6)
    Promoter
    hPGK
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247362-plasmid-map-sequence-id-491581

    Enlarge

  32. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

    Plasmid
    #246980
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    FLP Recombinase
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246980-plasmid-map-sequence-id-491526

    Enlarge

  33. pAAVLINK-EF1alpha-HA-5'-TCF20

    Plasmid
    #244284
    Purpose
    5' AAVLINK plasmid for TCF20 expression
    Depositor
    Zhonghua Lu
    Insert
    TCF20 (TCF20 Human)
    Use
    AAV
    Tags
    HA
    Promoter
    EF1alpha
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    244284-plasmid-map-sequence-id-491435

    Enlarge

  34. HiBiT-ALOX15 Fusion Vector

    Plasmid
    #238600
    Purpose
    Express HiBiT-ALOX15 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    ALOX15 (ALOX15 Human)
    Tags
    HiBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Oct. 28, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238600-plasmid-map-sequence-id-491575

    Enlarge

  35. pAAV-P3-Alb-mStayGold

    Plasmid
    #234549
    Purpose
    To produce AAV with the P3 promoter to express Alb(mouse)-mStayGold(Ando) fusion protein
    Depositor
    Hajime Hirase
    Article
    Knak et al Unpublished
    Insert
    Alb-mStayGold
    Use
    AAV
    Mutation
    None
    Promoter
    P3
    Available Since
    Oct. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234549-plasmid-map-sequence-id-471028

    Enlarge

  36. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

    Enlarge

  37. pTYB1-MBD2-N pc4789

    Plasmid
    #229753
    Purpose
    Bacterial expression plasmid of MBD2-N (N-terminus of MBD2: aa 1-152). C-terminal tagged to intein.
    Depositor
    Cristina Cardoso
    Article
    et al Unpublished
    Insert
    MBD2 (Mbd2 Mouse)
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    N-terminus of MBD2: aa 1-152
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229753-plasmid-map-sequence-id-460687

    Enlarge

  38. pTYB1-MBD4 pc4785

    Plasmid
    #229750
    Purpose
    Bacterial expression vector of MBD4 C-terminal tagged to the intein.
    Depositor
    Cristina Cardoso
    Article
    et al Unpublished
    Insert
    MBD4 (Mbd4 Mouse)
    Tags
    Intein
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229750-plasmid-map-sequence-id-458113

    Enlarge

  39. NanoLuc-PDPK1 Fusion Vector

    Plasmid
    #238841
    Purpose
    Express NanoLuc-PDPK1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PDPK1 (PDPK1 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238841-plasmid-map-sequence-id-481539

    Enlarge

Showing: 5001 - 5040 of 9324 results