We narrowed to 8,809 results for: CAG

1. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A APGST XE

   Plasmid

   #113848

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by T2A (APGST linker)

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-loxP-3xpA-sfGFP-tdTomato T2A APGST -XE

   Plasmid

   #113850

   Purpose

   Superfolder GFP and tdTomato linked by T2A (APGST linker, -XE)

   Depositor

   Mario Capecchi

   Insert

   sfGFP-T2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216246

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgAAVS1.5
   

   Use

   CRISPR; Human safe harbor locus (a avs1) site-spe…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216251

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgSEC61B.N1 (SEC61B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

   Plasmid

   #104567

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
   

   Use

   CRISPR

   Tags

   NLS-3xHA-VPR

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

   Plasmid

   #105323

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7c variant 1513
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

   Plasmid

   #114078

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

   Plasmid

   #157744

   Purpose

   knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   chlorotoxin containing chimeric antigen receptor targeting glioblastoma
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pA

   Plasmid

   #175438

   Purpose

   dual plasmid labelling cells of different neuronal population with different fluorophores

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   rox-STOP-rox-tRFP-pA
   

   Use

   Cre/Lox; Dre/rox

   Tags

   ZsGreen on backbone

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

    Plasmid

    #85041

    Purpose

    This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    loxP-stop-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid

    #180152

    Purpose

    tdTomato cre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

    Plasmid

    #199457

    Purpose

    Expression of dCas13-VPR in mammalian cells

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    dCas13
    

    Use

    Lentiviral

    Tags

    VPR-T2A-HaloTag

    Expression

    Mammalian

    Mutation

    Nuclease-dead H133A and H1058A

    Promoter

    CAG

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

    Plasmid

    #52419

    Purpose

    Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    ERAS (ERAS Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid

    #168106

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    mCherry-KASH
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

    Plasmid

    #68719

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

    Depositor

    Tobias Bonhoeffer, Mark Huebener, Tobias Rose

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid

    #68717

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

    Depositor

    Tobias Bonhoeffer, Mark Huebener, Tobias Rose

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cre-dependent expression from inverted open readi…

    Promoter

    CAG-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid

    #180156

    Purpose

    tdTomato cre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

    Plasmid

    #216321

    Purpose

    Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Luciferase + splice acceptor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216249

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    

    Use

    CRISPR; Endogenous tagging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

    Plasmid

    #196890

    Purpose

    Neuron-specific expression of human (h)Centrin fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen-hCentrin1 (CETN1 Human)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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