We narrowed to 5,557 results for: org

1. pST_DSUP_RAMVA (pBS0353)

   Plasmid

   #185189

   Purpose

   For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DSUP_RAMVA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-STIM1-NN_ST-SBP-mCherry

   Plasmid

   #65263

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)

   Plasmid

   #139445

   Purpose

   Lentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag E2-crimson
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6M-Pak1 H83L H86L

   Plasmid

   #12211

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   PBD (p21-binding domain) mutant: H83L, H86L (see …

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pASPIre1

   Plasmid

   #154842

   Purpose

   Derivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sites

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

   Insert

   Bxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Prha

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuide-Puro-GFPg1 (BB09)

   Plasmid

   #139461

   Purpose

   Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   GFP-targeting sgRNA inserted; resistance gene: puroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDY118A

   Plasmid

   #182958

   Purpose

   pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

   Depositor

   Shelley Copley

   Article

   Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

   Inserts

   Cas9
   Gam, Beta, Exo
   I-scel
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pASPIre3

   Plasmid

   #154844

   Purpose

   Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

   Insert

   Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pECFP-C1-hSERT

   Plasmid

   #70104

   Purpose

   mammalian expression of hSERT with N-terminal CFP fusion

   Depositor

   Harald Sitte

   Article

   Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

   Insert

   SERT (SLC6A4 Human)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-PH-SWAP70

    Plasmid

    #84582

    Purpose

    Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 residues 209-306 (SWAP70 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Pleckstrin-homology domain, residues 209-306

    Promoter

    CMV

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pOCC119

    Plasmid

    #118884

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease and mGFP, cl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. KTK_354

    Plasmid

    #180525

    Purpose

    KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ266

    Plasmid

    #164713

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiGuide-Neo-CTRLg2

    Plasmid

    #139451

    Purpose

    Lentiviral vector with non-targeting gRNA and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiGuide-Puro-CTRLg1 (CG509)

    Plasmid

    #139455

    Purpose

    Lentiviral vector with non-targeting gRNA and puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. TNF-SBP-mCherry_puromycin

    Plasmid

    #65285

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCellulose

    Plasmid

    #36300

    Depositor

    Jay Keasling

    Article

    Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

    Insert

    pCellulose
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Two promoters from the E coli genome drive two di…

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/mCherry-pBirA-HA

    Plasmid

    #154458

    Purpose

    expresses mCherry-pBirA-HA/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    mCherry-pBirA-HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F, T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-DONSON

    Plasmid

    #208797

    Purpose

    Xenopus laevis His6-Myc-DONSON wild type

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMTTH+H1.4ΔCTD

    Plasmid

    #157797

    Purpose

    Expression of human linker histone H1.4 without c-terminal tail

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H1.4ΔCTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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