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We narrowed to 8,664 results for: plat

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Showing: 2501 - 2520 of 8664 results

  2. qTAG-C-mStayGold-Puro-CANX

    Plasmid
    #227281
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CANX locus. For cilia visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CANX Homology Arms flanking a mStayGold-2A-Puro Cassette (CANX Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227281-plasmid-map-sequence-id-452050

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  3. pJAT21

    Plasmid
    #204309
    Purpose
    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPn
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204309-plasmid-map-sequence-id-405852

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  7. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Available Since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  8. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121817
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121817-plasmid-map-sequence-id-237365

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  10. WNT11-bio-His

    Plasmid
    #53430
    Purpose
    Expresses full-length Protein Wnt-11 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    WNT11 (WNT11 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53430-plasmid-map-sequence-id-83220

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  12. qTAG-C-Solo-mStayGold-COX8A

    Plasmid
    #227309
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227309-plasmid-map-sequence-id-451688

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  13. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid
    #227332
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227332-plasmid-map-sequence-id-452132

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  14. qTAG-N-Solo-mStayGold-ACTB

    Plasmid
    #227326
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227326-plasmid-map-sequence-id-452114

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  15. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid
    #227275
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227275-plasmid-map-sequence-id-452039

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  16. pJAT75

    Plasmid
    #204306
    Purpose
    Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204306-plasmid-map-sequence-id-406175

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  17. pJAT25

    Plasmid
    #204310
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPc
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204310-plasmid-map-sequence-id-405854

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  18. TFORF1483

    Plasmid
    #143123
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143123-plasmid-map-sequence-id-397868

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  19. TFORF1484

    Plasmid
    #143124
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143124-plasmid-map-sequence-id-397805

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  20. TFORF1480

    Plasmid
    #142802
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP63 (TP63 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142802-plasmid-map-sequence-id-395860

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Showing: 2501 - 2520 of 8664 results