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pGP-AAV-syn-jGCaMP7b-WPRE

Plasmid

#104489

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV Retrograde and AAV1

Insert

jGCaMP7b

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126)

Plasmid

#145839

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP7s-WPRE

Plasmid

#104487

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

GENIE Project, Douglas Kim

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP7s

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP5

Plasmid

#141259

Purpose

Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Has Service

Cloning Grade DNA

Insert

NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP12

Plasmid

#141265

Purpose

Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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I_pAAV-ProA6-CatCh-GFP-WPRE

Plasmid

#125890

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA6

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-p300 (D1399Y)

Plasmid

#191763

Purpose

Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

FRB-EGFP-p300 core (D1399Y) (EP300 Human)

Use

Tags

EGFP (N-terminal of p300 core (D1399Y)) and FRB (…

Expression

Bacterial and Mammalian

Mutation

catalytic D1399Y mutation in p300

Promoter

EF1alpha

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-ProA18-GCaMP6s

Plasmid

#126004

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

ProA18

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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198_pAAV-ProD1-CatCh-GFP-WPRE

Plasmid

#125977

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD1

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Rasgrf2-2A-dCre targeting vector

Plasmid

#61573

Purpose

Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Rasgrf2-2A-dCre (Rasgrf2 P1 bacteriophage, Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-3xGFP

Plasmid

#191204

Purpose

FlpO dependent GFP expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

3X GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-ProD1-GCaMP6s

Plasmid

#126005

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

ProD1

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6_nostop

Plasmid

#149309

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV2-ProC3-Cre/mCherry

Plasmid

#126006

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

Cre-2A-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

ProC3

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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70_pAAV-ProA23-CatCh-GFP-WPRE

Plasmid

#125906

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA23

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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VI_pAAV-ProA7-GFP-WPRE

Plasmid

#125891

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA7

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP5_nostop

Plasmid

#149308

Purpose

Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
136_pAAV-ProA1-CatCh-GFP-WPRE

Plasmid

#125886

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA1

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGASCD(K427E/K428E)

Plasmid

#127162

Purpose

expression of truncated human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Use

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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57_pAAV-ProA14-CatCh-GFP-WPRE

Plasmid

#125897

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA14

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge