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We narrowed to 8,237 results for: FIE

Showing: 5041 - 5080 of 8237 results

  1. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

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  2. psicheck_85_CA_repeat

    Plasmid
    #65881
    Purpose
    Luciferase assay vector, assessing regulation of 85 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 85 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pOpen-T7gene5

    Plasmid
    #165534
    Purpose
    DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    T7 DNA Polymerase (unmodified)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165534-plasmid-map-sequence-id-339124

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  4. Nup58_mEGFP(-7)

    Plasmid
    #163424
    Purpose
    Mammalian expression of Nup58 with a mEGFP (missing the first 7 amino acids) at amino acid position 412
    Depositor
    Sanford Simon
    Insert
    Nup58 (NUPL1 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    163424-plasmid-map-sequence-id-321894

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  5. pKT-H3.3-K27M-katushka

    Plasmid
    #209443
    Purpose
    SB-transposable plasmid expressing a mutant histone H3.3-K27M in tandem with Katushka (RFP)
    Depositor
    Maria Castro
    Insert
    H3.3-K27M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K27M
    Promoter
    Available since
    Jan. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    209443-plasmid-map-sequence-id-418361

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  6. pLVX-H3.3-G34V

    Plasmid
    #209433
    Purpose
    Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-G34V with BFP
    Depositor
    Maria Castro
    Insert
    H3.3-G34V
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G34V
    Promoter
    Available since
    Dec. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209433-plasmid-map-sequence-id-416707

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  8. pLSU1/t35s[]:nnLuz:NosT

    Plasmid
    #212183
    Purpose
    This binary vector expresses mushroom luciferase (nnLuz) with the truncated 35sCAMV promoter
    Depositor
    Wayne Curtis
    Insert
    nnLuz
    Use
    Tags
    Expression
    Plant
    Mutation
    codon modified for Physcomitrella patens expressi…
    Promoter
    truncated 35sCaMV
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212183-plasmid-map-sequence-id-443883

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  9. pKT-H3.3-G34R-IRES-katushka

    Plasmid
    #209428
    Purpose
    SB-transposable plasmid expressing the mutant histone H3.3-G34R with RFP
    Depositor
    Maria Castro
    Insert
    H3.3-G34R
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G34R
    Promoter
    Available since
    Jan. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    209428-plasmid-map-sequence-id-418354

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  10. psicheck_SRM1_3UTR

    Plasmid
    #65883
    Purpose
    Luciferase assay vector, assessing regulation of SRM1 3' UTR
    Depositor
    Thomas Tuschl
    Insert
    SRM1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. psicheck_50_100_CA_repeat

    Plasmid
    #65882
    Purpose
    Luciferase assay vector, assessing regulation of 50-100 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 50-100 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. Nup133_mEGFP(-8a)

    Plasmid
    #163417
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163417-plasmid-map-sequence-id-321553

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  13. pLVX-H3.3K27M-FLAG-BFP

    Plasmid
    #209434
    Purpose
    Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-K27M with BFP
    Depositor
    Maria Castro
    Insert
    H3.3-K27M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K27M
    Promoter
    Available since
    March 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    209434-plasmid-map-sequence-id-424833

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  14. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61972-plasmid-map-sequence-id-101285

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  15. pLenti_PGK_Prkaa1

    Plasmid
    #204356
    Purpose
    Lentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoter
    Depositor
    Günter Schneider
    Insert
    AMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    204356-plasmid-map-sequence-id-408696

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  16. B0030m_BC

    Plasmid
    #74394
    Purpose
    MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]
    Depositor
    Douglas Densmore
    Insert
    RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    74394-plasmid-map-sequence-id-140417

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  17. C35m

    Plasmid
    #120969
    Purpose
    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).
    Depositor
    Richard Murray
    Insert
    RhlR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BbsI sites removed; amino acid sequence …
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120969-plasmid-map-sequence-id-238056

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  19. Nup133_mEGFP(-9a)

    Plasmid
    #163419
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163419-plasmid-map-sequence-id-321638

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  21. pT2-3X-FLAG-H3.1-K27M-BFP

    Plasmid
    #209439
    Purpose
    SB-transposable plasmid expressing a FLAGGED mutant histone H3.1-K27M with BFP
    Depositor
    Maria Castro
    Insert
    H3.1-K27M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K27M
    Promoter
    Available since
    March 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    209439-plasmid-map-sequence-id-424832

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  22. Nup133_mEGFP(-9b)

    Plasmid
    #163420
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163420-plasmid-map-sequence-id-321639

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  23. Nup133_mEGFP(-8b)

    Plasmid
    #163418
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163418-plasmid-map-sequence-id-321635

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  24. pJK521

    Plasmid
    #72440
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Unspecified
    Tags
    Expression
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    n/a
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72440-plasmid-map-sequence-id-133778

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  25. psicheck_200_300_CA_repeat

    Plasmid
    #65880
    Purpose
    Luciferase assay vector, assessing regulation of 200-300 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 200-300 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. psicheck_300_400_CA_repeat

    Plasmid
    #65879
    Purpose
    Luciferase assay vector, assessing regulation of 300-400 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 300-400 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pLVX-EGFP-C3-cenexin

    Plasmid
    #73334
    Purpose
    Lentiviral vector encoding cenexin with an N-terminal EGFP tag
    Depositor
    Manuel Thery
    Insert
    cenexin/ODF2 (ODF2 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73334-plasmid-map-sequence-id-139040

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  28. pLVX-H3.3-G34R-FLAG-BFP

    Plasmid
    #209440
    Purpose
    Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-G34R with BFP
    Depositor
    Maria Castro
    Insert
    H3.3-G34R
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G34R
    Promoter
    Available since
    Nov. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    209440-plasmid-map-sequence-id-415312

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  29. pFRT-TODestFLAGHAhTTP

    Plasmid
    #48738
    Purpose
    Expresses hTTP
    Depositor
    Thomas Tuschl
    Insert
    TTP (ZFP36 Human)
    Use
    Tags
    FLAG, HA, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    48738-plasmid-map-sequence-id-70782

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  30. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI site removed
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

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  31. pLV-NT-3-mCherry

    Plasmid
    #190201
    Purpose
    To express the mouse NT-3 tagged with the FP mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    NT-3
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190201-plasmid-map-sequence-id-374466

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  33. pLXIN2-RFP

    Plasmid
    #99205
    Purpose
    RFP expressing bicistronic retroviral vector
    Depositor
    Saroj Mathupala
    Insert
    Red fluorescent protein
    Use
    Retroviral; Bicistronic retroviral expression vec…
    Tags
    Expression
    Mammalian
    Mutation
    The dsRed cDNA was PCR amplified off AddGene plas…
    Promoter
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99205-plasmid-map-sequence-id-192441

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  34. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Oliver Hobert
    Insert
    ceBFP SEC 3xFlag
    Use
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69340-plasmid-map-sequence-id-133241

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  35. Nup54-mEGFP494(0)

    Plasmid
    #163426
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163426-plasmid-map-sequence-id-321663

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  36. pCMV-PE2-pegRNA-tetra-com vector

    Plasmid
    #136271
    Purpose
    Expresses PE2 and Tetra-com modified pegRNA cassette
    Depositor
    Baisong Lu
    Insert
    PE2 and pegRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    136271-plasmid-map-sequence-id-273274

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  37. pCMV-PE2-pegRNA-ST2-com-vector

    Plasmid
    #136272
    Purpose
    Expresses PE2 and ST2-com modified pegRNA cassette
    Depositor
    Baisong Lu
    Insert
    PE2, pegRNA scaffold
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    136272-plasmid-map-sequence-id-273277

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  38. Nup54-mEGFP494(flex0)

    Plasmid
    #163429
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 5 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163429-plasmid-map-sequence-id-321671

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  39. Nup54-mEGFP494(flex2)

    Plasmid
    #163431
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 7 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163431-plasmid-map-sequence-id-321673

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  40. Nup54-mEGFP494(flex1)

    Plasmid
    #163430
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 6 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163430-plasmid-map-sequence-id-321672

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Showing: 5041 - 5080 of 8237 results