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Plasmid#132651PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 55 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjACTB-EGFP-5'short
Plasmid#129748PurposeGene targeting in marmoset cellsDepositorInsertACTB (ACTB C. jacchus (common marmost))
UseMarmoset targetingTagsEGFPExpressionMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.18_Ex_Ef1a-HA-EGFP-SV40-Zeo
Plasmid#128454Purposelentiviral expression vector with Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_S176E
Plasmid#131203Purposemammalian expression, tet RDepositorInsertRIPK2 S176E (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationS176EPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_3K
Plasmid#131205Purposemammalian expression, tet RDepositorInsertRIPK2 K209/410/538R (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationK209/410/538RPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_Y474F
Plasmid#131204Purposemammalian expression, tet RDepositorInsertRIPK2 Y474F (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationY474FPromoterpCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 VEGFA-TSS-guide4
Plasmid#125502PurposeCRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 VEGFA-TSS-guide2
Plasmid#125501PurposeCRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 VEGFA-TSS-guide1
Plasmid#125500PurposeCRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC801: pMVP (L3-L2) eGFP + WPRE
Plasmid#121771PurposepMVP L3-L2 entry plasmid, contains eGFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest in lentivirus vectors.DepositorInserteGFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO102: pMVP (R4-R3) eGFP open
Plasmid#121731PurposepMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteGFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP
Plasmid#121509PurposeExpresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).DepositorInsertsgFah.intron9
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IRES-EGFP-Flag-hRabin8
Plasmid#118073PurposeExpresses Flag-tagged human Rabin8 in mammalian cellsDepositorInserthuman Rabin8 (RAB3IP Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1 alphaAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant nmPKA-Cα-mEGFP
Plasmid#114144PurposeThe G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
UseTagsExpressionMammalianMutationG2A mutation and silent mutation to resist shRNA …PromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M4
Plasmid#114036PurposeExpression of HEC1 M4 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M4 (NDC80 Human)
UseTagsExpressionMammalianMutationM4 (K146E, R153E, K156E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M2
Plasmid#114035PurposeExpression of HEC1 M2 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M2 (NDC80 Human)
UseTagsExpressionMammalianMutationM2 (K81E, N87E, K89E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPC1_MTS_L4139P-eGFP-3HA
Plasmid#108404PurposeExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.DepositorInsertPKD1 (PKD1 Human)
UseTags3xHA and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mPC1_4119-eGFP-3HA
Plasmid#108393PurposeExpress cytoplasmic tail mouse PC1 from amino acid 4119 to 4293DepositorInsertPkd1 (Pkd1 Mouse)
UseTagseGFP-3HAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406A M407A
Plasmid#112107PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406A M407APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406R M407R
Plasmid#112109PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406R M407RPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_25-eGFP
Plasmid#107413PurposeThis plasmid contain promoter PthrC3_25, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPthrC3_25Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_7-eGFP
Plasmid#107412PurposeThis plasmid contain promoter PthrC3_7, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPthrC3_7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mPC1_4148-eGFP-3HA
Plasmid#108396PurposeExpress cytoplasmic tail mouse PC1 from amino acid 4148 to 4293DepositorInsertPkd1 (Pkd1 Mouse)
UseTagseGFP-3HAExpressionMammalianMutationvariant ID rs13468401 missense variant R4237QPromoterCMVAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/666E
Plasmid#105313PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/666EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/666A
Plasmid#105309PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/666APromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mEGFP
Plasmid#105143PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mEGFP
UseTags81nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG69-KcsA-Kv1.3-deGFP
Plasmid#102458PurposeExpresses KcsA-Kv1.3 protein with a C-terminal His tag and a deGFP fluorescent protein with a different ribosome binding site.DepositorArticleInsertsKcsA-Kv1.3
deGFP
UseTagsHis tagExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;snx5CE1-basEgfp
Plasmid#90138Purposecontains snx5 enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertsnx5E1basEgfp (snx5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bCE1-basEgfp
Plasmid#90136Purposecontains cldn5b enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertcldn5bE1basEgfp (cldn5b Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;yrkCE1-P1Egfp
Plasmid#90156Purposecontains yrk enhancer sequence, and yrk specific gene promoter driving expression of eGFPDepositorInsertyrkE1P1Egfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;snx5CE1-P1Egfp
Plasmid#90152Purposecontains snx5 enhancer sequence, and snx5 specific gene promoter driving expression of eGFPDepositorInsertsnx5E1P1Egfp (snx5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dusp5CE1-P1Egfp
Plasmid#90151Purposecontains dusp5 enhancer sequence, and dusp5 specific gene promoter driving expression of eGFPDepositorInsertdusp5E1P1Egfp (dusp5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;sheCE1-P1Egfp
Plasmid#90150Purposecontains She enhancer sequence, and She specific gene promoter driving expression of eGFPDepositorInsertsheE1P1Egfp (she Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;nrp1bCE1-P1Egfp
Plasmid#90149Purposecontains nrp1b enhancer sequence, and nrp1b specific gene promoter driving expression of eGFPDepositorInsertnrp1bE1P1Egfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dusp5CE1-basEgfp
Plasmid#90145Purposecontains dusp5 enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertdusp5E1basEgfp (dusp5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-basEgfp
Plasmid#90144Purposecontains dll4 enhancer 2 sequence and a basal promoter driving expression of eGFPDepositorInsertdll4E2basEgfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE1-basEgfp
Plasmid#90143Purposecontains dll4 enhancer 1 sequence and a basal promoter driving expression of eGFPDepositorInsertdll4E1basEgfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;yrkCE1-basEgfp
Plasmid#90142Purposecontains yrk enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertyrkE1basEgfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;lmo2CE1-basEgfp
Plasmid#90141Purposecontains lmo2 enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertlmo2E1basEgfp (lmo2 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;sheCE1-basEgfp
Plasmid#90139Purposecontains She enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertsheE1basEgfp (she Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only