We narrowed to 44,083 results for: cha

1. HpMscS

   Plasmid

   #78310

   Purpose

   H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

   Depositor

   Douglas Rees

   Article

   Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

   Insert

   Mechanosensitive channel of small conductance
   

   Use

   Tags

   6x His Tag and FLAG Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRTetR-GAL80

   Plasmid

   #185853

   Purpose

   Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT10R

   Plasmid

   #98307

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   TRPL3AcNES1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ERG20 N127W

   Promoter

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT2

   Plasmid

   #98306

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ERG20 N127W

   Promoter

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. HM3C NaVSp1_p pET24b

   Plasmid

   #127852

   Purpose

   Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NaVSp1
   

   Use

   Tags

   3C Cleavage site, His Tag, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

   Plasmid

   #52878

   Purpose

   Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

   Depositor

   Thomas Oertner

   Article

   Wietek et al Science. 2014 Mar 27.

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E90R, D156N, T159C

   Promoter

   CaMKIIa

   Available Since

   July 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. myc-SpELL pBacPAK8

   Plasmid

   #15318

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

   Insert

   eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
   

   Use

   Baculovirus construction

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. FLAG-SpEAF 60-251 pBacPAK8

   Plasmid

   #15321

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

   Insert

   EAF1 (eaf1 Fission Yeast)
   

   Use

   Baculovirus construction

   Tags

   FLAG

   Expression

   Mutation

   N terminal deletion lacking first 59 amino acids …

   Promoter

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. p11_AaC60αβ/C20

   Plasmid

   #229506

   Purpose

   Expresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20

   Depositor

   Laura Gunn

   Article

   Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

   Inserts

   Chaperonin 60α
   Chaperonin 60β
   Chaperonin 20
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N terminus chloroplast transit peptide truncation

   Promoter

   T7

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCUb-mVenus-pLJM

    Plasmid

    #236789

    Purpose

    MCUB tagged with mVenus for FRET / MW change for stoichiometry

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB (MCUB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJET-LB-geneticin

    Plasmid

    #213468

    Purpose

    Vector to amplify the LB-geneticin fragment for transformation into TSI locus 1

    Depositor

    Kim Hammond-Kosack

    Article

    Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

    Insert

    Left border of the TSI locus 1-split fragment of geneticin cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPG-GPD-TRPC

    Plasmid

    #190113

    Purpose

    Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

    Plasmid

    #153443

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMH-HT_ygiF-TTM

    Plasmid

    #127794

    Purpose

    Expresses the TTM/CYTH domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF (ygiF Escherichia coli)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 1-200)

    Promoter

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRPL28 SpHIS5 (pNTI663)

    Plasmid

    #115433

    Purpose

    Expresses wild-type RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRPL28(Q38P) SpHIS5 (pNTI664)

    Plasmid

    #115434

    Purpose

    Expresses Q38P RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q38P; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRPL28(H39L) SpHIS5 (pNTI665)

    Plasmid

    #115435

    Purpose

    Expresses H39L RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H39L; MfeI site in intron

    Promoter

    ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-His3MX6-PGAL1-GST

    Plasmid

    #41613

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJC25R1+4stop

    Plasmid

    #17723

    Depositor

    Susan Lindquist

    Article

    Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

    Insert

    Sup35R1+4 (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E.coli expression plasmid for NM-region of Sup35 …

    Promoter

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-SpELL pNMT-TOPO

    Plasmid

    #15315

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only