We narrowed to 42,612 results for: cha

1. PX458-3xHA-en31FnCas9

   Plasmid

   #201949

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en31FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   G1243T, E1369R, E1603H on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-3xHA-en1FnCas9

   Plasmid

   #201947

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en1FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1369R on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458-3xHA-en15FnCas9

   Plasmid

   #201948

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en15FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1603H on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)

   Plasmid

   #55614

   Purpose

   Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   C4762 mutated to serine to render HERC2 catalytic…

   Promoter

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCKO-CMV-TMEM106B-P2A-Blasticidin

   Plasmid

   #237478

   Purpose

   Encodes full length human TMEM106B protein, along with blasticidin selection marker.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B (TMEM106B Human)
   

   Use

   Lentiviral

   Tags

   P2A-Blasticidin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey

   Plasmid

   #238132

   Purpose

   This plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B
   

   Use

   Lentiviral

   Tags

   Blasticidin-P2A-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster

   Plasmid

   #238131

   Purpose

   This plasmid encodes the full-length hamster (Mesocricetus auratus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B
   

   Use

   Lentiviral

   Tags

   Blasticidin-P2A-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458-3xHA-FnCas9ABEmax8.17d

   Plasmid

   #201955

   Purpose

   Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RPL25-GFP (94)

   Plasmid

   #24037

   Depositor

   Michael Rout

   Insert

   RPL25 (RPL25 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

10. RPS1B-GFP (95)

    Plasmid

    #24039

    Depositor

    Michael Rout

    Insert

    RPS1B (RPS1B Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

11. RPL3-GFP (96)

    Plasmid

    #24040

    Depositor

    Michael Rout

    Insert

    RPL3 (RPL3 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

12. RPL25NLS-GFP (101)

    Plasmid

    #24038

    Depositor

    Michael Rout

    Insert

    RPL25 (RPL25 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Only bp 1-135 (aa 1-45) of RPL25

    Promoter

    Available since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

13. PHO4NLS-GFP-PRA (109)

    Plasmid

    #24044

    Depositor

    Michael Rout

    Insert

    PHO4
    

    Use

    Tags

    EGFP and PrA

    Expression

    Yeast

    Mutation

    Only bp 421-588 of PHO4 (NLS)

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

14. NAB2NLS-GFP-PRA (107)

    Plasmid

    #24043

    Depositor

    Michael Rout

    Insert

    NAB2
    

    Use

    Tags

    EGFP and PrA

    Expression

    Yeast

    Mutation

    Only bp 601-750 of NAB2 (NLS)

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

15. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid

    #111903

    Purpose

    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.

    Depositor

    Andrew Carter

    Inserts

    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    

    Use

    Tags

    8xHis, SNAPf-tag, TEV site, and ZZ-tag

    Expression

    Insect

    Mutation

    Optimised for expression in Sf9 cells.

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. RPL25NLS-GFP-PRA (102)

    Plasmid

    #24041

    Depositor

    Michael Rout

    Insert

    RPL25 (RPL25 Budding Yeast)
    

    Use

    Tags

    EGFP and PRA

    Expression

    Yeast

    Mutation

    Only bp 1-135 (aa 1-45) of RPL25

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

17. KAP95-FLU (113)

    Plasmid

    #24045

    Depositor

    Michael Rout

    Insert

    KAP95 (KAP95 Budding Yeast)
    

    Use

    Tags

    FLU

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2010

    Availability

    Academic Institutions and Nonprofits only

18. KAP123-FLU (116)

    Plasmid

    #24048

    Depositor

    Michael Rout

    Insert

    KAP123 (KAP123 Budding Yeast)
    

    Use

    Tags

    FLU

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. KAP121-FLU (115)

    Plasmid

    #24047

    Depositor

    Michael Rout

    Insert

    KAP121 (PSE1 Budding Yeast)
    

    Use

    Tags

    FLU

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pLCKO-CMV-ACE2-P2A-Blasticidin

    Plasmid

    #237476

    Purpose

    Encodes full length human ACE2 protein, along with blasticidin selection marker.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    P2A-Blasticidin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge