We narrowed to 10,315 results for: plasmids 101

1. AAVS1-Blasticidin XLone-eGFP

   Plasmid

   #159754

   Purpose

   AAVS1 donor plasmid for targeted inducible eGFP expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Insert

   all-in-one tet-on system
   

   Use

   CRISPR and TALEN; Donor plasmid for targeted knoc…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS inducible promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Ef1a-Con/Fon-mCherry

   Plasmid

   #137132

   Purpose

   Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

   Plasmid

   #159107

   Purpose

   The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

   Depositor

   Thomas Tuschl

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   NSP12
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-nEF-Coff/Fon-iC++-EYFP

   Plasmid

   #137157

   Purpose

   Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-iC++-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Ef1a-Con/Foff 2.0-EYFP

   Plasmid

   #137162

   Purpose

   Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-nEF-Con/Fon/Von eYFP

   Plasmid

   #137164

   Purpose

   Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV Retrograde and AAV8

   Insert

   3x-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-nEF-Con/Fon-iC++-EYFP

   Plasmid

   #137155

   Purpose

   Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-iC++-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-Ef1a-Con/Fon-sRGECO

   Plasmid

   #137126

   Purpose

   Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-sRGECO
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E217D

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-Ef1a-Coff/Fon-GCaMP6M

   Plasmid

   #137121

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Ef1a-Coff/Fon-BFP

    Plasmid

    #137131

    Purpose

    Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid

    #110624

    Purpose

    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    LP1B and hU6

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Ef1a-Con/Fon-BFP

    Plasmid

    #137129

    Purpose

    Intersectional viral expression of BFP in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Ef1a-Con/Foff 2.0-mCherry

    Plasmid

    #137133

    Purpose

    Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

    Plasmid

    #176060

    Purpose

    Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K164A and H165A

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

    Plasmid

    #176059

    Purpose

    Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Ef1a-Coff/Fon-oScarlet

    Plasmid

    #137138

    Purpose

    Intersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Ef1a-Coff/Fon-sRGECO

    Plasmid

    #137128

    Purpose

    Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-sRGECO
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E217D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

    Plasmid

    #137165

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    3x-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Ef1a-Con/Foff 2.0-BFP

    Plasmid

    #137130

    Purpose

    Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

    Plasmid

    #127867

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Human, Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    constructed by blunt end fusion of the 5’ 51 nucl…

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge