We narrowed to 6,748 results for: tac

1. pDMG8c

   Plasmid

   #33181

   Depositor

   David Bartel

   Article

   Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

   Insert

   C27H6.9 3'UTR (C27H6.9 Nematode)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   LTA sites: atacGaTa, atacGaTa

   Promoter

   Available since

   Jan. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pDMG1c

   Plasmid

   #33160

   Depositor

   David Bartel

   Article

   Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

   Insert

   cog-1 3'UTR (cog-1 Nematode)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   LTA sites: atacGaTa, atacGaTa

   Promoter

   Available since

   Jan. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pDMG4c

   Plasmid

   #33169

   Depositor

   David Bartel

   Article

   Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

   Insert

   ptp-1 3'UTR (ptp-1 Nematode)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   ] LTA sites: tacGaTa, atacGaTa

   Promoter

   Available since

   Jan. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pBABE-hygro-mTurquoise2-Nuf2

   Plasmid

   #80762

   Purpose

   Express Nuf2 N-terminally labeled with mTurquoise2

   Depositor

   Daniel Needleman

   Article

   Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

   Insert

   Nuf2 (NUF2 Human)
   

   Use

   Retroviral

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMY01AB-PRO

   Plasmid

   #127714

   Purpose

   Integrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMY01CD-TER

   Plasmid

   #127751

   Purpose

   Integrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX208 CMV sTurboID (C)-HA-FRB-ERM

   Plasmid

   #153007

   Purpose

   Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)-ERM (Cb5)
   

   Use

   Lentiviral

   Tags

   FRB and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

   Plasmid

   #153006

   Purpose

   Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   OMM (Tom20)-sTurboID (N) (L73)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSP2-96-merTetO-EFS-BLaR

   Plasmid

   #118713

   Purpose

   Contains an optimized 96-mer TetO repeat for imaging of desired loci

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Inserts

   Blasticidin resistance gene
   EFS-NS promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSP2-48-merTetO-EFS-BLaR

    Plasmid

    #118712

    Purpose

    Contains an optimized 48-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Dcp1a

    Plasmid

    #153972

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCh-Rab7A

    Plasmid

    #61804

    Purpose

    Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP

    Plasmid

    #220075

    Purpose

    expresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBP

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL5 H478/9A (OSBPL5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H478/9A, deltaTMD

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ADAM10-HA

    Plasmid

    #65106

    Purpose

    Expression of HA tagged ADAM10 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPR-tagBFP

    Plasmid

    #75160

    Purpose

    Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-KG-D4H*-mCherry

    Plasmid

    #134604

    Purpose

    fluorescent cholesterol-binding probe

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    D4H*
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    Y415A and A463W

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. tdTomato-β-actinCDS3

    Plasmid

    #183715

    Purpose

    tdTomato with fragment nucleotides 801-1127 of beta-actin coding sequence inserted in 3'UTR

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Insert

    tdTomato with fragment (nucleotides 801-1127) of mouse Actb coding dsequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-Mfn1

    Plasmid

    #141154

    Purpose

    Mammalian expression and labeling mitofusin 1

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Mitofusin 1 (MFN1 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

    Plasmid

    #153005

    Purpose

    Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID-high affinity (C) (G79)
    

    Use

    Lentiviral

    Tags

    FRB, HA, and HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

    Plasmid

    #153004

    Purpose

    Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID-high affinity (N) (G78)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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