We narrowed to 12,673 results for: sequence

1. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-KDEL_neomycin

   Plasmid

   #65306

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
   

   Use

   Tags

   KDEL motif and Signal Sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLminP_Luc2P_RE27

   Plasmid

   #90369

   Purpose

   Type II Interferon - STAT1/STAT1 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   STAT1

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGK 4xMito-mEmerald

   Plasmid

   #200430

   Purpose

   Mammalian expression of fluorescently-tagged marker for mitochondria

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   4x mito-targeting sequence

   Promoter

   PGK

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pG108-K - AhpC-Glow

   Plasmid

   #185135

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Inserts

   Glow gene
   Bacteroides thetaiotaomicron ahpC promoter
   

   Use

   Pg106

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-NES-KIRIN1

   Plasmid

   #107416

   Purpose

   Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CROPseq-Puro-24xMS2

   Plasmid

   #212629

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ePE2

   Plasmid

   #202029

   Purpose

   Prime Editing plasmid for tag insertion

   Depositor

   Pengcheng Wei

   Article

   Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

   Insert

   Cas9，
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-myc3-NEDD8

   Plasmid

   #19943

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

10. AAV-CAG-flex-MCS--4x6T

    Plasmid

    #196420

    Purpose

    Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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