We narrowed to 9,334 results for: bli

1. pAAV-hSyn-TeTxLC-P2A-mTurquoise2

   Plasmid

   #239210

   Purpose

   Expresses tetanus toxin light chain in neurons to inhibit synapse activity

   Depositor

   Erin O'Shea

   Insert

   TeTxLC-P2A-mTurquoise2
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQdCas12a.sggfp(A)

   Plasmid

   #236038

   Purpose

   The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
   

   Use

   CRISPR

   Promoter

   quorum sensing promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQdCas12a.sggfp(B)

   Plasmid

   #236040

   Purpose

   The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
   

   Use

   CRISPR

   Promoter

   quorum sensing promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRDA_852

   Plasmid

   #185527

   Purpose

   Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Inserts

   First sgRNA cassette
   Second sgRNA cassette
   

   Use

   CRISPR, Cre/Lox, and Lentiviral ; Flp/frt

   Expression

   Mammalian

   Promoter

   Human U6 and Mouse U6

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pdCas9-tdTomato

   Plasmid

   #232631

   Purpose

   catalytically inactive dCas9 for localization; labeled with tdTomato

   Depositor

   Karsten Rippe

   Insert

   dCas9-tdTomato
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE

   Plasmid

   #231805

   Purpose

   Label the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.

   Depositor

   Annette Oxenius

   Insert

   eGFP-mCEP83
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti puro HA-STX3

   Plasmid

   #227590

   Purpose

   Lentiviral plasmid expressing human STX3 protein

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   STX3 (STX3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330-MM002-ZFP143-C-terminal-sgRNA

   Plasmid

   #212706

   Purpose

   Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

   Depositor

   Elzo de Wit

   Insert

   ZFP143 C-terminal sgRNA (Zfp143 Mouse)
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR/hBIG1

   Plasmid

   #226251

   Purpose

   Entry plasmid of hBIG1 for Gateway system

   Depositor

   Hye-Won Shin

   Insert

   BIG1 (ARFGEF1 Human)
   

   Use

   Entry vector

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR/HA-hBIG2

    Plasmid

    #226252

    Purpose

    Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system

    Depositor

    Hye-Won Shin

    Insert

    BIG2 (ARFGEF2 Human)
    

    Use

    Entry vector

    Tags

    HA

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti puro HA-VIM

    Plasmid

    #227585

    Purpose

    Lentiviral plasmid expressing human VIM protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    VIM (VIM Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

    Plasmid

    #225432

    Purpose

    Transcriptional unit of BeYDV Rep/RepA (C1:C2)

    Depositor

    Diego Orzaez

    Insert

    pNOS:Rep/RepA:tNOS
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEN_TT DNM1L-V5

    Plasmid

    #220359

    Purpose

    Gateway entry vector for an inducible V5-tagged DNM1L

    Depositor

    Kevin Janes

    Insert

    DNM1L (DNM1L Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    V5

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3.SLC17A2-flag

    Plasmid

    #224419

    Purpose

    Expresses SLC17A2 in mammalian cells

    Depositor

    Jonathan Long

    Insert

    Solute carrier family 17 (sodium phosphate), member 2 (SLC17A2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CD55-Delta3

    Plasmid

    #220872

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-3
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-3, short consensus repeat (SCR) domains 1, …

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CD55-Delta4

    Plasmid

    #220873

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-4
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-4, short consensus repeat (SCR) domains 1, …

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CD55-Delta1

    Plasmid

    #220870

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-1
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-1, the first short consensus repeat (SCR) d…

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CD55-Delta2

    Plasmid

    #220871

    Purpose

    Expresses CD55 variant in mammalian cells

    Depositor

    Ferenc Scheeren

    Insert

    CD55 Delta-2
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Delta-2, short consensus repeat (SCR) domains 1 a…

    Promoter

    CMV

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hs-cre::EBD, UAS-dBrainbow attB

    Plasmid

    #220326

    Purpose

    expresses a heat-shock inducible cre::EBD fusion protein and UAS-dBrainbow

    Depositor

    Erdem Bangi

    Inserts

    Cre::EBD (with intron)
    dBrainbow
    

    Expression

    Insect

    Promoter

    UAS and hs

    Available Since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDF-B-pC

    Plasmid

    #213495

    Purpose

    Encodes Node B of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    cinI (cinI )
    lacI (lacI )
    lambda cI (lambdap50 )
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and LVA tag

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYES2/NTA/Leu-LegA7Δank

    Plasmid

    #216519

    Purpose

    Expression of LegA7 ΔAnk

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 331-454

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYES2/NTA/Leu-LegA7ΔAnk290

    Plasmid

    #216520

    Purpose

    Expression of LegA7 ΔAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔAnk290
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-454

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYES2/NTA/Leu-LegA7ΔNAnk264

    Plasmid

    #216521

    Purpose

    Expression of LegA7 ΔNAnk264

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk264
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 264-361

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYES2/NTA/Leu-LegA7ΔNAnk290

    Plasmid

    #216522

    Purpose

    Expression of LegA7 ΔNAnk290

    Depositor

    Ralph Isberg

    Insert

    LegA7 ΔNAnk290
    

    Tags

    HIS-tag

    Expression

    Bacterial and Yeast

    Mutation

    LegA7 missing aa 290-361

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-RPL3L-TwStrep

    Plasmid

    #208649

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949

    Depositor

    Quanfu Ma

    Insert

    human RPL3L (ENST00000268661) (RPL3L Human)
    

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    contain rs753338989, rs746044959, rs752134949, pr…

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-CAG-K-red-SPOTIT(R271F)

    Plasmid

    #191494

    Purpose

    Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    K-red-SPOTIT(R271F)
    

    Use

    AAV

    Mutation

    R271F in OR

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-CAG-M-red-SPOTIT2(R279F)

    Plasmid

    #191495

    Purpose

    Red fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    M-red-SPOTIT2(R279F)
    

    Use

    AAV

    Mutation

    R279F in OR

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET50_G495R MDA5

    Plasmid

    #216803

    Purpose

    Expresses a single point mutant of melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Expression

    Bacterial

    Mutation

    G495R

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTRIPZ-NUDT17-C-HA-Puro

    Plasmid

    #195182

    Purpose

    Lentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.

    Depositor

    Stephanie Roessler

    Insert

    NUDT17 (NUDT17 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV promoter and minimal CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7CFE1_polio_3ABC_C147R

    Plasmid

    #212985

    Purpose

    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus Type I Mahoney 3ABC C147R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    cysteine 147 in 3C changed to arginine

    Promoter

    T7

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT7CFE1_EVA71_VP12A_C110A

    Plasmid

    #212976

    Purpose

    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Enterovirus A71 4643 VP12A C110A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA tag

    Mutation

    changed cysteine 110 from 2A to alanine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pT7CFE1_polio_VP12A_C109R

    Plasmid

    #212987

    Purpose

    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus Type I Mahoney VP12A C109R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    Cysteine 109 from 2A mutated to arginine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCHA_SBV_NSs

    Plasmid

    #208938

    Purpose

    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    NSs
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 47 bp at 5' end and 510 bp from 3&#x…

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28-Flag-RBM24-95~236

    Plasmid

    #208634

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 95~236, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAGW-Fray

    Plasmid

    #203979

    Purpose

    GFP-Fray under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Frayed (fray Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAMW-WnkAAxV

    Plasmid

    #203980

    Purpose

    Myc6 Wnk under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    6xMyc

    Expression

    Insect

    Mutation

    RFxV mutated to AAxV

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lenti-Cas9-Exo-Blast-BFP

    Plasmid

    #196721

    Purpose

    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-Exo1-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGB-06-07-Vps34

    Plasmid

    #187989

    Purpose

    Plasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323

    Depositor

    Sascha Martens

    Insert

    Vps34 (PIK3C3 Human)
    

    Expression

    Bacterial and Insect

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hSyn-Aion-mKate2

    Plasmid

    #192574

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid

    #185061

    Purpose

    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

    Depositor

    Bobby Koeleman

    Insert

    U6:pegRNA:scaffold:PBS+RT template
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge