We narrowed to 5,479 results for: KRAS

1. pCMV-PE2-tagRFP-BleoR

   Plasmid

   #192508

   Purpose

   Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

   Depositor

   Gerald Schwank

   Article

   Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   PE2-P2A-tagRFP-P2A-BleoR
   

   Tags

   BleoR, SV40 bpNLS, and tagRFP

   Expression

   Mammalian

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNH605-HSE-YFP

   Plasmid

   #172131

   Purpose

   Fluorescent reporter for Hsf1 activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xHSE-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Expression

   Yeast

   Promoter

   CYC1 core promotor with 4 tandem repeats of the H…

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5FRT/TO-Strep/HA-UBE2J1

   Plasmid

   #124669

   Purpose

   Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2J1 (UBE2J1 Human)
   

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HaloTag-WDR5

   Plasmid

   #200880

   Purpose

   Mammalian expression of human WDR5 with N-terminal HaloTag fusion

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   HaloTag-WDR5 (WDR5 Human)
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1-Neurod1-3xT7

   Plasmid

   #200890

   Purpose

   Inducible lentiviral expression of Neurod1

   Depositor

   Kai Ge

   Article

   Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

   Insert

   Neurod1 (Neurod1 Mouse)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   3xT7

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR221-YAP1

   Plasmid

   #79503

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   FLAG

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. DRH335: FCK-Optopatch1

   Plasmid

   #51695

   Purpose

   Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   Optopatch1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pProEx Rap1A(G12C, L96F) CL

   Plasmid

   #224264

   Purpose

   Protein purification of Rap1A (G12C, L96F)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rap1A (RAP1A Human)
   

   Expression

   Bacterial

   Mutation

   G12C, L96F

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. DNMT3L-dCas9

    Plasmid

    #126587

    Purpose

    DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

    Insert

    DNMT3L (DNMT3L Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-DNMT3L-dCas9-NLS

    Expression

    Mammalian

    Available Since

    Dec. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNH605-PACE-YFP

    Plasmid

    #172002

    Purpose

    Fluorescent reporter for Rpn4 activity

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    4xPACE-YFP
    

    Use

    Vector for genomic integration into the yeast gen…

    Expression

    Yeast

    Promoter

    CYC1 core promotor with 4 tandem repeats of Rpn4 …

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9 TadCBEa-V106W

    Plasmid

    #193838

    Purpose

    Express TadCBEa-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa V106W-SpCas9 D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2CG2-acta1:QFGal4-SV40pA

    Plasmid

    #155121

    Purpose

    Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    acta1:QFGal4
    

    Use

    Zebrafish expression

    Promoter

    acta1

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

    Plasmid

    #172514

    Purpose

    Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
    

    Use

    AAV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

    Plasmid

    #159689

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

    Depositor

    Dominic Esposito

    Article

    Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2T-cmv-SpCas9-TadCBEa-BlastR

    Plasmid

    #193847

    Purpose

    Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEa
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FLEX-SaCas9-U6-sgGria2

    Plasmid

    #124854

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CMV-cGAS C396/7A-HA

    Plasmid

    #130917

    Purpose

    Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS C396/7A
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutated residues 396 and 397 to alanines

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5FRT/TO-VCPIP1-Strep-HA

    Plasmid

    #113482

    Purpose

    Expression of human VCPIP1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    VCPIP1 (VCPIP1 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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