We narrowed to 13,780 results for: TIM

1. pRSETA-qmTQ2-ATP

   Plasmid

   #225247

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-ATP
   

   Use

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

   Plasmid

   #175526

   Purpose

   Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag11 and Tomm20-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. RGS-6xHis-pcDNA3.1-

   Plasmid

   #52534

   Purpose

   enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Type

   Empty backbone

   Use

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBES1-LacOx50-Hyg

   Plasmid

   #210261

   Purpose

   Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   50xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. dimericTomato-1xwGBD

   Plasmid

   #176099

   Purpose

   Visualization of active Cdc42

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

   Insert

   WAS (WAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMVdel

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMito-Electra2

   Plasmid

   #184927

   Purpose

   Mitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8A

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   COX8A-Electra2
   

   Use

   Tags

   Electra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1-Nup35

   Plasmid

   #87342

   Purpose

   To express human Nup35 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   nucleoporin 35 (NUP35 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP-NTEVp(WT)

   Plasmid

   #137831

   Purpose

   Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-NTEVp(WT)
   

   Use

   Tags

   3x FLAG and NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. FRB-CTEVp(WT)

   Plasmid

   #137832

   Purpose

   Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FRB-CTEVp(WT)
   

   Use

   Tags

   3x FLAG and NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. piggyBAC-5xTAPE-1-BlastR

    Plasmid

    #175808

    Purpose

    Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Insert

    5xTAPE-1 construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p414-TEF1p-Cas9-CYC1t

    Plasmid

    #43802

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJ915

    Plasmid

    #69090

    Purpose

    Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

    Depositor

    Jennifer Doudna

    Article

    Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

    Insert

    cas9
    

    Use

    Tags

    MBP, hexa His, and two SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1X-Nup93

    Plasmid

    #87336

    Purpose

    To express human Nup93 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP93 (NUP93 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

    Plasmid

    #163077

    Purpose

    Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Peredox
    

    Use

    Gateway entry vector

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV_Stargazin-mTurquoise2-iLID

    Plasmid

    #161001

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    Stargazin-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMVdel-dimericTomato-WASp(CRIB)

    Plasmid

    #191450

    Purpose

    Visualizing endogenous & active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    WASp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFC330

    Plasmid

    #87842

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. SGEN

    Plasmid

    #111171

    Purpose

    miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    SFFV

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTrc-trGPPS(CO)-LS

    Plasmid

    #50603

    Purpose

    pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

    Depositor

    Jay Keasling

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Inserts

    Limonene Synthase
    ApR
    LacIq
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    truncated codon optimized geranylpyrophosphate sy…

    Promoter

    Trc

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAJM.657

    Plasmid

    #108525

    Purpose

    CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    CymRAM + PCymRC-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CymR: S110G A171V

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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